Zinc (Zn) is an essential micronutrient to plants, but in most of the vineyard soils, its amount surpasses the threshold value recommended by the European Union. With this work, we intended to evaluate the growth, morpho-histological traits and nucleolar activity in leaves and roots of three grapevines (Vitis vinifera L.) varieties (‘Donzelinho Branco’, ‘Verdelho’ and ‘Bastardo’). Forced cuttings of those varieties were placed in hydroponic solutions supplemented with 1.5, 3 and 5 mM Zn for two weeks to extrapolate their tolerance to Zn. Hoagland's nutritive solution (0 mM Zn) was used as control. The Zn treatments stimulated the leaf growth in all varieties, confirmed by the increase of the leaf mitotic index (MI). Leaf growth was higher in ‘Donzelinho Branco’ treated with 5 mM Zn, but it was accompanied by a significant reduction of the leaf thickness. The increase of Zn concentration contributed to the roots thickening and the decrease of the root MI, mean nucleolar number and area. The inhibition of root elongation and the leaf and root nucleolar activity changes seen in the Zn-treated plants are compatible with osmotic stress responses. The integration of the multidisciplinary data of this work allowed us to suggest that ‘Verdelho’ and ‘Donzelinho Branco’ were more tolerant than ‘Bastardo’ to higher Zn concentrations.

锌 (Zn) 是植物必需的微量营养素，但在大多数葡萄园土壤中，其含量超过欧盟推荐的阈值。通过这项工作，我们打算评估三种葡萄树（Vitis viniferaL.) 品种（“Donzelinho Branco”、“Verdelho”和“Bastardo”）。将这些品种的强制插条置于补充有 1.5、3 和 5 mM Zn 的水培溶液中两周，以推断它们对 Zn 的耐受性。Hoagland 的营养溶液 (0 mM Zn) 用作对照。锌处理刺激了所有品种的叶片生长，这通过叶片有丝分裂指数 (MI) 的增加得到证实。用 5 mM Zn 处理的 'Donzelinho Branco' 的叶子生长更高，但伴随着叶子厚度的显着降低。Zn浓度的增加导致根系变粗，根系MI、平均核仁数和面积减少。在锌处理植物中观察到的根伸长抑制以及叶和根核仁活性变化与渗透胁迫反应相容。