Abstract

Understanding the interaction between oral keratinocytes (NOK-si) and Candida albicans is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml⁻¹, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and post hoc tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and C. albicans and increases in the CFU ml⁻¹ values of C. albicans biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by C. albicans biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of C. albicans. It was concluded that the interaction of cells in co-culture influenced their dynamics over time.

摘要

了解口腔角质形成细胞 (NOK-si) 和白色念珠菌之间的相互作用是制定口腔念珠菌病预防策略和新疗法的基础。该研究通过细胞代谢、CFU ml ^{-1 的}数量以及酶、细胞因子和代谢物的产生来评估在共培养中生长的这些细胞的动力学和代谢特征。数据通过方差分析和事后检验（α = 0.05）进行分析。在共培养物中，有在NOK-Si和的细胞代谢显著下跌白色念珠菌和增加在CFU毫升^-1的值的白色念珠菌生物膜。在它们相互作用后，NOK-si 产生的细胞因子和白色念珠菌生物膜产生的蛋白酶也显着增加。主要代谢物、氨基酸以及细胞外和细胞内代谢物的代谢平衡发生了有利于共培养的变化，而白色念珠菌的生物膜会分泌更多的芳香醇。得出的结论是，共培养中细胞的相互作用会随着时间的推移影响它们的动态。