Abstract

The sensitive determination of pesticides constitutes an important aspect in ensuring food safety. Due to the disadvantages of traditional methods, such as low sensitivity, complex operation, and high cost, the development of an efficient and economical biosensor to detect pesticide residues is required. In this work, the dipeptide glycyl-L-histidine (GH) and divalent copper ions (Cu²⁺) were employed as bioorganic linkers and metal ions in the synthesis of the peptide-based metal organic framework (MOF) material GH-Cu BioMOF. Because of its properties of fluorescence quenching and adsorption of nucleic acids, GH-Cu BioMOF was used to develop a distinctive aptamer based method for the determination of pesticide residues in food. The GH-Cu BioMOF-based aptasensor was used for acetamiprid and demonstrated a linear response in the range from 0.15 to 3.00 µM with a detection limit of 137 nM, verifying the feasibility of this method for pesticide determination in food.

摘要

农药的灵敏测定是确保食品安全的重要方面。由于传统方法灵敏度低、操作复杂、成本高等缺点，需要开发一种高效、经济的农药残留检测生物传感器。在这项工作中，二肽甘氨酰-L-组氨酸 (GH) 和二价铜离子 (Cu ²⁺) 在合成肽基金属有机框架 (MOF) 材料 GH-Cu BioMOF 中用作生物有机接头和金属离子。由于其荧光猝灭和核酸吸附特性，GH-Cu BioMOF 被用于开发一种独特的基于适体的食品中农药残留测定方法。基于 GH-Cu BioMOF 的适体传感器用于啶虫脒，在 0.15 至 3.00 µM 范围内表现出线性响应，检测限为 137 nM，验证了该方法用于食品中农药测定的可行性。