As an essential part in the toolbox of super-resolution microscopy, stimulated emission depletion (STED) nanoscopy has been widely explored in revealing the substructure and bioactivities in fluorescence imaging. Among the applied STED fluorophores, silicon-substituted rhodamines (SiRs) belong to one of the most extensively employed fluorophores. The carboxy-SiR was favored in STED bioimaging with many advantages, including reliable photostability, cell permeability, tunable fluorogenicity, feasible structural decoration and so on. We reviewed the research of carboxy-SiR in the STED nanoscopy and hopefully this can inspire more efforts in the design and application of STED fluorophores.

作为超分辨率显微镜工具箱中的重要组成部分，受激发射损耗 (STED) 纳米显微镜在揭示荧光成像中的子结构和生物活性方面得到了广泛的探索。在应用的 STED 荧光团中，硅取代的罗丹明 (SiR) 属于应用最广泛的荧光团之一。羧基-SiR具有可靠的光稳定性、细胞渗透性、可调节的荧光性、可行的结构装饰等优点，在STED生物成像中受到青睐。我们回顾了羧基-SiR 在 STED 纳米显微镜中的研究，希望这可以激发更多的 STED 荧光团设计和应用的努力。