The vertical migration of second-stage juveniles (J2) of Meloidogyne floridensis and M. incognita was investigated in segmented 14-cm long PVC soil columns having their top ring in contact with soil of a potted plant. Both bottom and top rings were screened appropriately to allow only upward nematode movement and preventing root penetration from the potted plant into the soil in the column. Plants used for nematode’s stimuli were the nematode attractive tomato (Solanum lycopersicum L.) ‘Cobra’ and the nematode repellent French marigold (Tagetes patula L.) var. Petite. Host-free columns were used as control. Columns with and without plants were placed in growth chambers at 20 °C and inoculated with each root-knot nematode species by injecting 1000 J2 through a hole in the basal ring of each column. Columns were dismantled at 3, 6 and 9 days after injection and J2 were extracted from soil of each ring and the planted pot; additionally, roots in the top pot were stained to observe J2 penetration. No preferential upward migration towards either plant stimuli was observed, but M. floridensis was more migrant than M. incognita, with 4.4% and 1.6% of active J2, respectively, reaching an upward distance of more than 13 cm regardless of plant stimuli. This study supports random migration of root-knot nematode J2, even without stimulus from a host plant.

佛罗里达根结线虫和南方根结线虫的第二阶段幼虫 (J2) 的垂直迁移在分段的 14 厘米长 PVC 土柱中进行了研究，这些土柱的顶环与盆栽植物的土壤接触。底部和顶部环都经过适当筛选，以仅允许线虫向上移动并防止根从盆栽植物渗透到柱子中的土壤中。用于线虫刺激的植物是吸引线虫的番茄 ( Solanum lycopersicum L.) 'Cobra' 和驱除线虫的法国万寿菊 ( Tagetes patulaL.) 变种。娇小。无宿主柱用作对照。将带有和不带有植物的柱子放置在 20 °C 的生长室中，并通过通过每个柱子基环中的孔注射 1000 J2 接种每种根结线虫物种。注射后第3、6、9天拆除柱子，从每圈土壤和种植盆中提取J2；此外，对顶盆中的根进行染色以观察 J2 渗透。没有观察到任何植物刺激物的优先向上迁移，但M. floridensis比M. incognita更具迁移性，分别有 4.4% 和 1.6% 的活性 J2，无论植物刺激如何，都能达到超过 13 cm 的向上距离。这项研究支持根结线虫 J2 的随机迁移，即使没有来自寄主植物的刺激。