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Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-07-01 , DOI: 10.1111/mmi.14780
Nathan P Wasilko 1 , Josue S Ceron 1 , Emily R Baker 1 , Andrew G Cecere 1 , Michael S Wollenberg 2 , Tim I Miyashiro 1
Affiliation  

Sulfur is in cellular components of bacteria and is, therefore, an element necessary for growth. However, mechanisms by which bacteria satisfy their sulfur needs within a host are poorly understood. Vibrio fischeri is a bacterial symbiont that colonizes, grows, and produces bioluminescence within the light organ of the Hawaiian bobtail squid, which provides an experimental platform for investigating sulfur acquisition in vivo. Like other γ-proteobacteria, V. fischeri fuels sulfur-dependent anabolic processes with intracellular cysteine. Within the light organ, the abundance of a ΔcysK mutant, which cannot synthesize cysteine through sulfate assimilation, is attenuated, suggesting sulfate import is necessary for V. fischeri to establish symbiosis. Genes encoding sulfate-import systems of other bacteria that assimilate sulfate were not identified in the V. fischeri genome. A transposon mutagenesis screen implicated YfbS as a sulfate importer. YfbS is necessary for growth on sulfate and in the marine environment. During symbiosis, a ΔyfbS mutant is attenuated and strongly expresses sulfate-assimilation genes, which is a phenotype associated with sulfur-starved cells. Together, these results suggest V. fischeri imports sulfate via YfbS within the squid light organ, which provides insight into the molecular mechanisms by which bacteria harvest sulfur in vivo.

中文翻译:

费氏弧菌在与 Eurymna scolopes 共生过程中输入和同化硫酸盐

硫存在于细菌的细胞成分中,因此是生长所必需的元素。然而,人们对细菌在宿主内满足其硫需求的机制知之甚少。费氏弧菌是一种细菌共生体,在夏威夷短尾鱿鱼的光器官内定殖、生长并产生生物发光,为研究体内硫获取提供了实验平台。与其他 γ-变形菌一样,费氏弧菌通过细胞内半胱氨酸为硫依赖性合成代谢过程提供燃料。在光器官内,不能通过硫酸盐同化合成半胱氨酸的ΔcysK突变体的丰度减弱,这表明硫酸盐输入对于V. fischeri是必要的建立共生关系。在费氏弧菌基因组中没有发现编码其他吸收硫酸盐的细菌的硫酸盐输入系统的基因。转座子诱变筛选表明 YfbS 作为硫酸盐进口商。YfbS 是在硫酸盐和海洋环境中生长所必需的。在共生过程中,ΔyfbS突变体减弱并强烈表达硫酸盐同化基因,这是与硫饥饿细胞相关的表型。总之,这些结果表明V. fischeri通过 squid light 器官内的 YfbS 输入硫酸盐,这提供了对细菌在体内收获硫的分子机制的深入了解。
更新日期:2021-07-01
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