Anaesthesia exposure causes changes in the developing brain and affects behaviour and memory. This study examined the beneficial effect of pachypodol against isoflurane (ISF)-induced neuronal injury. Seven-day-old rats were treated with 10 mg/kg and 30 mg/kg intravenous pachypodol 30 min before exposure to ISF (0.75%) for 6 h. Oxidative stress and other biochemical parameters were assessed in the brain tissue and serum using enzyme-linked immunosorbent assay. Additionally, a terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay was performed to assess neuronal cell apoptosis in several regions of the hippocampus. Cognitive function and neurological scores were determined in the pachypodol-treated neuron-injured rats. Cytokine levels and oxidative stress were reduced in the pachypodol-treated group compared with the ISF group. In addition, cognitive deterioration was reversed in pachypodol-treated compared with ISF-treated rats. Thus, treatment with pachypodol reduced neuronal apoptosis in neuron-injured rats. Moreover, pachypodol ameliorated changes to the JNK/ERK/Akt pathway in brain-injured rats. In conclusion, pachypodol treatment prevents neuronal apoptosis in ISF-treated rats by regulating the JNK/ERK pathway.

中文翻译：

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Pachypodol 通过调节 JNK/ERK 通路保护新生大鼠免受麻醉诱导的发育中脑细胞凋亡

麻醉暴露会导致发育中的大脑发生变化并影响行为和记忆。本研究检查了茯苓多酚对异氟醚 (ISF) 诱导的神经元损伤的有益作用。7 日龄大鼠在暴露于 ISF (0.75%) 6 小时前 30 分钟用 10 mg/kg 和 30 mg/kg 静脉注射茯苓多酚处理。使用酶联免疫吸附试验评估脑组织和血清中的氧化应激和其他生化参数。此外，进行末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 测定以评估海马几个区域的神经元细胞凋亡。在多酚处理的神经元损伤大鼠中测定认知功能和神经学评分。与 ISF 组相比，pachypodol 治疗组的细胞因子水平和氧化应激降低。此外，与 ISF 处理的大鼠相比，pachypodol 处理的认知恶化被逆转。因此，用芍药醇治疗可减少神经元损伤大鼠的神经元凋亡。此外，茯苓醇改善了脑损伤大鼠 JNK/ERK/Akt 通路的变化。总之，pachypodol 治疗通过调节 JNK/ERK 通路来预防 ISF 治疗大鼠的神经元凋亡。