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Characterization of siderophore-producing microorganisms associated to plants from high-Andean heavy metal polluted soil from Callejón de Huaylas (Ancash, Perú)
Microbiological Research ( IF 6.7 ) Pub Date : 2021-07-02 , DOI: 10.1016/j.micres.2021.126811
Carmen Tamariz-Angeles 1 , Gabriela D Huamán 2 , Edson Palacios-Robles 2 , Percy Olivera-Gonzales 1 , Alberto Castañeda-Barreto 1
Affiliation  

Endophytic and rhizospheric microorganisms associated with six native plants adapted to heavy metal polluted soil from Punta Olímpica and Chahuapampa, located in Callejón de Huaylas mountains, were evaluated as potential candidates for technologies to clean polluted ecosystems. It was selected 14 bacteria and 9 fungi strains by their iron and/or aluminum siderophore production trait, where BEP17-Dm showed higher production. According to the 16S rDNA analysis, bacteria belong to Pseudomonas, Bacillus, and Achromobacter genera, whereas by ITS analysis fungi belong to Talaromyces, Hypoxylon, Tolypocladium, and Penicillium. All bacteria strains tolerated lead (2−8 mM) and eigth tolerated cadmium (1−6 mM); also all fungi tolerated lead (9−70 mM) and cadmium (3−10 mM). Two bacteria and six fungi solubilized cadmium carbonate, while eleven bacteria and two fungi solubilized tricalcium phosphate, where P. japonica BEP18-Dm and B. subtilis BRU16-Sr exhibited higher solubilization index. None strains solubilized lead carbonate. BEP18-Dm produced higher concentration of IAA (53.42 μgml−1); while six bacteria and all fungi strains produced a low concentration of auxins. Medicago sativa seedlings inoculated with BEP17-Dm, BEP18-Dm, or BRU16-Sr showed more surviving percentage under in vitro culture in presence of Cd, Pb (0.5–1.0 mM), or Al (2.5–5.0 mM). Finally, it is the first report of siderophore-producing microorganisms from polluted soil of Callejón de Huaylas highlands, interestedly they displayed metabolic properties useful to enhance phytoremediation and biotechnology application.



中文翻译:

与来自 Callejón de Huaylas(秘鲁安卡什)的高安第斯重金属污染土壤中的植物相关的产铁微生物的特征

与位于 Callejón de Huaylas 山脉的 Punta Olímpica 和 Chahuapampa 的六种本地植物相关的内生和根际微生物被评估为清洁污染生态系统技术的潜在候选者。根据铁和/或铝铁载体生产性状筛选出 14 种细菌和 9 种真菌菌株,其中 BEP17-Dm 显示出较高的产量。根据 16S rDNA 分析,细菌属于假单胞菌属芽孢杆菌属和无色杆菌属,而通过 ITS 分析真菌属于踝节菌属HypoxylonTolypocladium青霉菌属. 所有细菌菌株均耐受铅 (2−8 mM) 和第八种耐受镉 (1−6 mM);还所有真菌耐受铅 (9-70 mM) 和镉 (3-10 mM)。2种细菌和6种真菌溶解了碳酸镉,11种细菌和2种真菌溶解了磷酸三钙,其中P. japonica BEP18-Dm和B. subtilis BRU16-Sr表现出较高的溶解指数。没有菌株溶解碳酸铅。BEP18-Dm 产生更高浓度的 IAA (53.42 μgml -1 );而六种细菌和所有真菌菌株产生低浓度的生长素。接种 BEP17-Dm、BEP18-Dm 或 BRU16-Sr 的苜蓿幼苗在体外显示出更高的存活率在存在 Cd、Pb (0.5–1.0 mM) 或 Al (2.5–5.0 mM) 的情况下进行培养。最后,这是 Callejón de Huaylas 高地污染土壤中产生铁载体微生物的第一份报告,有趣的是,它们显示了有助于增强植物修复和生物技术应用的代谢特性。

更新日期:2021-07-07
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