This study investigated the effects of zinc oxide nanoparticles (ZnON) on growth performance, gut health, and zinc and copper excretion in piglets. A total of 144 piglets (Duroc × Landrace × Large White, weaned at 21 days; mean initial weight 6.15 ± 0.01 kg) were assigned to six groups with six replicates of four pigs per pen for a 26-day feeding trial. The groups were: antibiotic group (basal diet + 75 mg/kg aureomycin), ZnO group (basal diet + 1,600 mg/kg zinc oxide), and four ZnON groups (basal diet + 200, 300, 400, or 500 mg/kg ZnON, respectively). No significant difference in growth performance was observed amongst the treatments. ZnON supplementation improved the activities of superoxide dismutase (linear or quadratic, p < 0.05), copper-zinc superoxide dismutase in the ileum (linear, p < 0.05), catalase in the jejunum and ileum (linear or quadratic, p < 0.05), total antioxidant capacity of duodenum and ileum (linear, p < 0.05), and glutathione peroxidase in the ileum (linear, p < 0.05), and reduced malondialdehyde content in the jejunum (linear, p < 0.05). As ZnON content was increased, the contents of jejunal IgA, interleukin (IL)-6, and IL-10 decreased (linear, p < 0.05), while IgM content increased (linear, p < 0.05). The contents of ileal IgA and IL-10 increased (linear and quadratic, p < 0.05), while the contents of IgM, IgG, and IL-6 decreased (linear or quadratic, p < 0.05). Villus height and the ratio of villus height to crypt depth in piglets fed with ZnON were higher than in the antibiotic group (p < 0.05). Pigs fed with 200–500 mg/kg ZnON showed lower contents of hepatic zinc and copper and faecal zinc than the ZnO group (p < 0.05). These results show that dietary ZnON enhanced intestinal antioxidant and immune capacity and intestinal morphology, particularly 500 mg/kg ZnON, and also that ZnON minimised zinc content in the liver and faeces.

本研究调查了氧化锌纳米颗粒 (ZnO N) 对仔猪生长性能、肠道健康以及锌和铜排泄的影响。总共 144 头仔猪（杜洛克 × 长白 × 大白猪，在 21 天断奶；平均初始体重 6.15 ± 0.01 kg）被分配到六个组，每栏四头猪六次重复，用于 26 天的饲养试验。各组为：抗生素组（基础饮食 + 75 mg/kg 金霉素）、ZnO 组（基础饮食 + 1,600 mg/kg 氧化锌）和四个 ZnO N 组（基础饮食 + 200、300、400 或 500 mg/ kg ZnO N）。在处理之间没有观察到生长性能的显着差异。ZnO N 补充提高了超氧化物歧化酶的活性（线性或二次，p < 0.05)，回肠中的铜锌超氧化物歧化酶（线性，p  < 0.05），空肠和回肠中的过氧化氢酶（线性或二次，p  < 0.05），十二指肠和回肠的总抗氧化能力（线性，p  < 0.05）和回肠中的谷胱甘肽过氧化物酶（线性，p  < 0.05），并降低空肠中的丙二醛含量（线性，p  < 0.05）。随着 ZnO N 含量的增加，空肠 IgA、白细胞介素 (IL)-6 和 IL-10 的含量降低（线性，p  < 0.05），而 IgM 含量增加（线性，p  < 0.05）。回肠 IgA 和 IL-10 的含量增加（线性和二次曲线，p < 0.05)，而 IgM、IgG 和 IL-6 的含量降低（线性或二次，p  < 0.05）。饲喂ZnO N 的仔猪的绒毛高度和绒毛高度与隐窝深度的比率高于抗生素组（p  < 0.05）。饲喂 200-500 mg/kg ZnO N 的猪的肝脏锌、铜和粪便锌含量低于 ZnO 组（p  < 0.05）。这些结果表明，膳食 ZnO N 增强了肠道抗氧化和免疫能力以及肠道形态，尤其是 500 mg/kg ZnO N，而且 ZnO N 最大限度地减少了肝脏和粪便中的锌含量。