Turbidity is a common pollutant parameter in water and wastewater treatment that can be reduced through flocculation. This research was conducted to characterise and analyse the potential of bioflocculant-producing bacteria isolated from aquaculture effluent. Samples were taken from two different aquaculture farms that represent brackish and freshwater cultures. A total of 21 different colonies were obtained from all samples. Screening of flocculation activity showed the potential of WS5 to be a bioflocculant agent to remove turbidity from artificial turbid water. One variable at a time analysis was performed to assess the capability of WS5 in different initial turbidity and bioflocculant doses. In comparison with low turbid water with 5% v/v bioflocculant dose (in 5000 NTU turbidity), WS5 exhibited better performance in high turbid water, thereby resulting in the most optimum flocculation activity of 72.5%. The increment in bioflocculant dose of up to 10% did not contribute to the flocculation activity significantly. The increment in particle size distribution for the suspension from before to after treatment with bioflocculant provided evidence that the process of flocculation had occurred effectively. Genomic analysis of selected potential bacteria using BLAST and phylogenetic tree has identified WS5 as Serratia marcescens and has been confirmed as a rod-shaped bacterium. FT-IR spectra and zeta potential analysis indicated that the produced bioflocculant is categorised as anionic bioflocculant with polymer bridging suggested as the main mechanism during turbidity removal.

浊度是水和废水处理中常见的污染物参数，可通过絮凝降低。本研究旨在表征和分析从水产养殖废水中分离出的产生生物絮凝剂的细菌的潜力。样品取自代表咸水和淡水养殖的两个不同水产养殖场。从所有样品中总共获得了 21 个不同的菌落。絮凝活性的筛选表明 WS5 具有作为生物絮凝剂去除人工混浊水浊度的潜力。进行一次一个变量分析以评估 WS5 在不同初始浊度和生物絮凝剂剂量下的能力。与 5% v/v 生物絮凝剂剂量（5000 NTU 浊度）的低浊水相比，WS5 在高浊水中表现出更好的性能，从而产生 72.5% 的最佳絮凝活性。生物絮凝剂剂量增加高达 10% 对絮凝活性没有显着贡献。悬浮液在生物絮凝剂处理前后粒径分布的增加提供了絮凝过程有效发生的证据。使用 BLAST 和系统发育树对选定的潜在细菌进行基因组分析，已将 WS5 鉴定为Serratia marcescens并已被确认为杆状细菌。FT-IR 光谱和 zeta 电位分析表明，所生产的生物絮凝剂属于阴离子型生物絮凝剂，聚合物桥联被认为是除浊过程中的主要机制。