The Schistosoma japonicum fatty acid-binding protein (FABP) is used in the cell membrane to absorb and transport fatty acids, which cannot be resynthesized by the organism and combined with hydrophobic ligands. Among the 5 stages of the worm life cycle examined, FABP messenger ribonucleic acid (mRNA) expression was highest in male adult worms, followed by the liver-stage schistosome, and was the lowest in the lung-stage schistosome. The fabp gene-coding region was cloned and expressed to obtain recombinant S. japonicum FABP (rSjFABP) with a molecular weight of approximately 18 kDa. Mice were then immunized against rSjFABP to prepare anti-FABP serum. Using immunohistochemical techniques, FABP protein was found to localize to the eggshell, parenchyma, and digestive tract. Double-stranded RNA-mediated knockdown of FABP mRNA by RNA interference decreased the number of transcripts by >70%. Moreover, the egg production rate decreased, whereas the abnormal egg ratio was significantly increased in the FABP-silenced group compared with the negative control group (P < 0.05). These results demonstrate that FABP localizes in adults and in various stages. FABP contributes to the egg-laying capacity of adults, which may be related to the reproductive function of S. japonicum.

在日本血吸虫脂肪酸结合蛋白（FABP）是在细胞膜中用于吸收和运输的脂肪酸，其不能由生物体再合成，并用疏水性配体结合。在检查的蠕虫生命周期的 5 个阶段中，FABP 信使核糖核酸 (mRNA) 表达在雄性成虫中最高，其次是肝期血吸虫，在肺期血吸虫中最低。所述FABP基因编码区被克隆并表达，以获得重组日本血吸虫FABP（R Sj的具有大约18 kDa的分子量FABP）。然后针对 r Sj对小鼠进行免疫FABP 制备抗 FABP 血清。使用免疫组织化学技术，发现 FABP 蛋白定位于蛋壳、薄壁组织和消化道。通过 RNA 干扰双链 RNA 介导的 FABP mRNA 敲低使转录本数量减少了 70% 以上。此外，与阴性对照组相比，FABP沉默组产蛋率降低，而异常产蛋率显着增加（P < 0.05）。这些结果表明 FABP 定位于成人和不同阶段。FABP有助于成虫的产卵能力，这可能与S. japonicum的生殖功能有关。