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Alteration of unfolded protein responses and autophagy signaling represented the molecular basis underlying saccharin toxicity to Drosophila (Diptera: Drosophilidae)
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2021-07-01 , DOI: 10.1002/arch.21826
Angelina F Osabutey 1 , A-Young Kim 2 , Bo Yoon Seo 3 , Linh Xuan Mai 1 , Young Ho Koh 1, 2
Affiliation  

The purpose of this study was to develop a new control method for Drosophila using saccharin sodium dihydrate (saccharin), an artificial sweetener that is safe for humans and the environment, and to elucidate its mode of action. In this study, we confirmed that saccharin can dose-dependently inhibit the development of or kill vinegar flies (VFs) and spotted wing Drosophila (SWDs). In addition, we found that low concentrations of saccharin induced a similar effect as starvation in Drosophila, whereas high concentrations of saccharin induced changes in the unfolded protein response (UPR) and autophagy signaling that were unlike starvation and inhibited development or killed the VF and the SWD by performing real-time quantitative polymerase chain reaction analyses. Spinosad is a widely used plant protection agent for SWD control. When saccharin was cotreated with 0.25–1.0 ppm spinosad, an additive insecticidal activity was observed only at high concentrations of saccharin. However, when saccharin was cotreated with 2.0 ppm spinosad, an additive insecticidal activity was observed at low concentrations of saccharin. Taken together, alteration of UPR and autophagy signaling represented the molecular basis underlying saccharin toxicity to Drosophila and concurrent spraying of an insecticide with saccharin could enhance the insecticidal activities.

中文翻译:

未折叠蛋白反应和自噬信号的改变代表了糖精对果蝇(双翅目:果蝇科)毒性的分子基础

本研究的目的是开发一种新的果蝇控制方法,使用二水糖精钠(糖精),一种对人类和环境安全的人造甜味剂,并阐明其作用模式。在这项研究中,我们证实糖精可以剂量依赖性地抑制或杀死醋蝇(VF)和斑点翅果蝇(SWD) 的发育。此外,我们发现低浓度的糖精在果蝇中引起与饥饿相似的效果,而高浓度的糖精诱导未折叠蛋白反应 (UPR) 和自噬信号的变化,这与饥饿不同,并通过实时定量聚合酶链反应分析抑制发育或杀死 VF 和 SWD。多杀菌素是一种广泛用于 SWD 控制的植物保护剂。当糖精与 0.25-1.0 ppm 多杀菌素共同处理时,仅在高浓度糖精下观察到附加的杀虫活性。然而,当糖精与 2.0 ppm 多杀菌素共同处理时,在低浓度的糖精下观察到了附加的杀虫活性。总之,UPR 和自噬信号的改变代表了糖精对果蝇的毒性的分子基础 杀虫剂与糖精同时喷洒可增强杀虫活性。
更新日期:2021-07-18
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