ABSTRACT

The steady increase in the employment of silver nanoparticles (AgNPs) in consumer products entails the determination of the aquatic toxicity of AgNPs. Various AgNP characteristics including particle size, and shape, surface charge, and material have prominent effects on ecotoxicity. In the present study, we investigated the aquatic toxicity of chemically-synthesized AgNPs (Che-AgNPs) and green synthesis AgNPs (Gr-AgNPs) to Daphnia magna as a model organism. In each case, Che-AgNPs and Gr-AgNPs showed dose-dependent toxicity in the range of 5–50 ppb. It was also detected that the size and surface coverage material of AgNPs has a significant impact on the survival rate of D. magna. We also analyzed the expression of some genes related to detoxification and the reproductive system. These observations presented that in both NP types the significant alterations were detected in genes of the model organism in a dose-dependent manner.

摘要

消费产品中银纳米粒子 (AgNPs) 使用的稳步增加需要确定 AgNPs 的水生毒性。各种 AgNP 特性，包括粒径、形状、表面电荷和材料，对生态毒性具有显着影响。在本研究中，我们研究了化学合成的 AgNPs (Che-AgNPs) 和绿色合成 AgNPs (Gr-AgNPs)作为模式生物对大型蚤的水生毒性。在每种情况下，Che-AgNPs 和 Gr-AgNPs 都表现出 5-50 ppb 范围内的剂量依赖性毒性。还检测到 AgNPs 的尺寸和表面覆盖材料对D. magna的存活率有显着影响. 我们还分析了一些与解毒和生殖系统相关的基因的表达。这些观察表明，在两种 NP 类型中，模式生物的基因中都以剂量依赖性方式检测到显着变化。