Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.

中文翻译：

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苹果C2H2型锌指转录因子MdZAT10通过与MdBT2相互作用正调控JA诱导的叶片衰老

茉莉酸（JA）在调节叶片衰老中起重要作用。然而，苹果叶片衰老的分子机制（海棠) 仍然难以捉摸。在本研究中，我们发现苹果中的 C2H2 型锌指转录因子 (TF) MdZAT10 显着加速叶片衰老并增加衰老相关基因的表达。为了探索 MdZAT10 如何促进叶片衰老，我们进行了液相色谱/质谱筛选。我们发现 MdABI5 与 MdZAT10 在物理上相互作用。MdABI5是一种重要的叶片衰老正调节因子，显着加速了苹果叶片的衰老。发现 MdZAT10 可增强 MdABI5 的转录活性MdNYC1和MdNYE1，从而加速叶片衰老。此外，我们发现MdZAT10茉莉酸甲酯 (MeJA) 诱导表达，加速了 JA 诱导的叶片衰老。我们还发现 JA 反应蛋白 MdBT2 直接与 MdZAT10 相互作用，并通过泛素化和降解降低其蛋白质稳定性，从而延缓 MdZAT10 介导的叶片衰老。总之，我们的研究结果为 MdZAT10 正向调节 JA 诱导的苹果叶片衰老的机制提供了新的见解。