DNA extraction is of utmost importance in archaeobiology, as it determines the success of further DNA analyses. This study concentrates on the success of ancient DNA extraction using silica spin columns and PCR-based analysis from archaeological skeletal material and investigates the influence of sample quantity, lysis time and lysis temperature during sample preparation. The results show that lysis times ranging from 2 to 48 h are suitable, and that lysis should be carried out at a constant temperature of 56°C. Concerning sample quantity, 10 mg for mitochondrial DNA and 50 mg for chromosomal DNA are sufficient for high quality analyses. Thus invaluable sample material can be saved, and time of sample preparation can be reduced considerably.

中文翻译：

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样本数量和裂解参数对从骨骼遗骸中提取古 DNA 成功的影响。

DNA 提取在考古生物学中至关重要，因为它决定了进一步 DNA 分析的成功与否。本研究的重点是使用硅胶离心柱和基于 PCR 的考古骨骼材料分析成功提取古 DNA，并研究样品制备过程中样品数量、裂解时间和裂解温度的影响。结果表明，裂解时间为2~48 h是合适的，裂解应在56°C恒温下进行。关于样品量，10 mg 线粒体 DNA 和 50 mg 染色体 DNA 足以进行高质量分析。因此可以节省宝贵的样品材料，并可以大大减少样品制备时间。