Chitosan and its derivatives have numerous applications in wastewater treatment as bio-coagulants, flocculants and bio-adsorbents against both particulate and dissolved pollutants. Chitinolytic bacteria secrete an array of enzymes, which play crucial role in chitin to chitosan conversion. Consequently, there is a growing demand for identification and characterization of novel bacterial isolates with potential implications in chitosan production. We describe genomic features of the new isolate Streptomyces sp. UH6. Analysis of the 6.51 Mb genome revealed the GC content as 71.95% and presence of 6990 coding sequences of which 63% were functionally annotated. Further, we identified two possible chitin-utilization pathways, which employ secreted enzymes like lytic polysaccharide monooxygenases and family-18 glycoside hydrolases (GHs). More importantly, the genome has six family-4 polysaccharide deacetylases with probable role in chitin to chitosan conversion, as well as two chitosanases belonging to GH46 and GH75 families. In addition, the gene clusters, dasABC and ngcEFG coding for transporters, which mediate the uptake of N,N′-diacetylchitobiose and N-acetyl-d-glucosamine were identified. Several genes responsible for hydrolysis of other polysaccharides and fermentation of sugars were also identified. Taken together, the phylogenetic and genomic analyses suggest that the isolate Streptomyces sp. UH6 secretes potential chitin-active enzymes responsible for chitin to chitosan conversion.

中文翻译：

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链霉菌属的基因组分析。UH6 揭示了对壳聚糖生产至关重要的潜在几丁质分解机制的存在

壳聚糖及其衍生物在废水处理中作为生物混凝剂、絮凝剂和生物吸附剂具有许多应用，可对抗颗粒污染物和溶解污染物。几丁质分解细菌分泌一系列酶，这些酶在几丁质向壳聚糖的转化中起着至关重要的作用。因此，对对壳聚糖生产具有潜在影响的新型细菌分离物的鉴定和表征的需求不断增长。我们描述了新分离链霉菌的基因组特征sp。UH6。对 6.51 Mb 基因组的分析显示 GC 含量为 71.95%，并且存在 6990 个编码序列，其中 63% 被功能注释。此外，我们确定了两种可能的几丁质利用途径，它们采用分泌酶，如裂解多糖单加氧酶和家族 18 糖苷水解酶 (GHs)。更重要的是，该基因组有 6 个家族 4 多糖脱乙酰酶，可能在几丁质向壳聚糖的转化中起作用，以及 2 个属于 GH46 和 GH75 家族的壳聚糖酶。此外，基因簇dasABC和ngcEFG编码转运蛋白，介导N、N' - 二乙酰壳二糖和N - 乙酰 - d-氨基葡萄糖被鉴定。还鉴定了几个负责其他多糖水解和糖发酵的基因。总之，系统发育和基因组分析表明分离株链霉菌属。UH6 分泌潜在的几丁质活性酶，负责几丁质向壳聚糖的转化。