ABSTRACT

M-dichlorobenzene can be degraded through biotrickling filter (BTF). However, its degradation needs to be enhanced, and the mechanism of enhancement is unclear. In this study, Zn(II) was taken as the biological growth promoter; the polysaccharides and proteins, zeta potential, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, and the enzyme activity of the strains were analyzed. The results indicated that Zn(II) at 5mg/L promoted the growth of the strain. When the inlet concentration was 505.74 mg/m³ and the empty bed resistance time (EBRT) was 90 s, the removal efficiency of BTF2 was 86.15%. In BTF2, the enzyme activity, polysaccharide, and protein content increased, while the zeta potential decreased. The changes of zeta potential indicated that there existed an electrostatic interaction between extracellular polymeric substance (EPS) and Zn(II). The results of Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy confirmed that there was a bond between the EPS surface functional groups and Zn(II).

摘要

间二氯苯可通过生物滴滤器 (BTF) 降解。但其降解需要增强，增强机制尚不清楚。本研究以Zn(II)为生物促生长剂；对菌株的多糖和蛋白质、zeta电位、傅里叶变换红外光谱、X射线光电子能谱和酶活性进行了分析。结果表明5mg/L的Zn(II)促进了菌株的生长。当入口浓度为 505.74 mg/m ^{3 时}空床阻力时间（EBRT）为90 s，BTF2去除率为86.15%。在BTF2中，酶活性、多糖和蛋白质含量增加，而zeta电位降低。Zeta电位的变化表明胞外聚合物(EPS)与Zn(II)之间存在静电相互作用。傅里叶变换红外光谱和 X 射线光电子能谱的结果证实 EPS 表面官能团与 Zn(II) 之间存在键。