Herbicides may pose considerable danger to non-target aquatic organisms and further threaten human health. The present investigation was aimed to assess the effects of 2-methyl-4-chlorophenoxy acetic acid (MCPA-Na) on Cyprinus carpio embryos. Embryos were exposed to six concentrations of MCPA-Na (0, 52, 54, 56, 58 and 60 mg/L) for 96 h. A series of symptoms were observed in developmental embryos during MCPA-Na exposure, including increased death, hatching inhibited and morphological deformities. Further, MCPA-Na exposure leading to a series of morphological changes (pericardial edema, tail deformation, and spine deformation) in embryos, which were consistent with modifications in the associated genes. In this work, we also investigated the joint toxicity of herbicides (MCPA-Na and cyhalofop-butyl) commonly used in paddy fields on carp embryos, using the 96 h-LC₅₀ of herbicides (59.784 mg/L MCPA-Na and 1.472 mg/L cyhalofop-butyl) and confirmed that a synergistic effect existing in the binary mixtures.

除草剂可能对非目标水生生物构成相当大的危险，并进一步威胁人类健康。本研究旨在评估 2-甲基-4-氯苯氧基乙酸 (MCPA-Na) 对鲤鱼的影响胚胎。胚胎暴露于六种浓度的 MCPA-Na（0、52、54、56、58 和 60 毫克/升）96 小时。在 MCPA-Na 暴露期间，在发育胚胎中观察到一系列症状，包括死亡增加、孵化受抑制和形态畸形。此外，MCPA-Na 暴露导致胚胎的一系列形态变化（心包水肿、尾部变形和脊柱变形），这与相关基因的修饰一致。在这项工作中，我们还使用 96 h-LC ₅₀的除草剂（59.784 mg/L MCPA-Na 和 1.472 mg/L MCPA-Na 和 1.472 mg /L cyhalofop-butyl) 并证实二元混合物中存在协同效应。