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Antithrombotic effects of Huanglian Jiedu decoction in a rat model of ischaemia-reperfusion-induced cerebral stroke
Pharmaceutical Biology ( IF 3.8 ) Pub Date : 2021-07-01 , DOI: 10.1080/13880209.2021.1942505
Huan Liu 1 , Xiaoyan Chen 1 , Yanling Liu 1 , Chunjuan Fang 1 , Shaofen Chen 1
Affiliation  

Abstract

Context

Huanglian Jiedu Decoction (HLJJD) has a variety of pharmacological activities, such as anti-inflammatory and neuroprotection against ischaemic brain injury.

Objectives

This ex vivo study explores its antithrombosis activity and inhibition of platelet aggregation.

Material and methods

To study the antithrombosis activity of HLJJD ex vivo, saline, or HLJDD (100, 200, and 500 mg/kg) was treated prophylactically by gavage for 3 days in Wistar rats (n = 4). Based on the rat model of transient middle cerebral artery infarction (MCAO) or normal rats (n = 4), the antithrombotic activity in the normal group and HLJDD subgroups on prothrombin time, thrombus weight, platelet aggregation, and others was evaluated, followed by the antiplatelet aggregation of its main components (n = 4).

Results

The weight of the thrombus increased significantly at 24 h after MCAO onset. HLJJD did not influence the change of PT, but significantly inhibited thrombosis by 12.5, 20.0, and 20.5% in reducing the dry weight of thrombus, and blocked collagen-induced platelet aggregation by 25.5, 39.0, and 42.7% and adhesion of blood platelet by 17.3, 26.2, and 27.3%. The IC50 value of HLJJD on collagen-induced platelet aggregation was 670 mg/kg. Geniposide only facilitated antiplatelet aggregation induced by collagen, but not AA or ADP. Both baicalin and berberine showed markedly antiplatelet aggregation induced by all activators. The antithrombotic activity of baicalin was relatively higher than that of berberine (35.0–47.8% vs. 20.6–33.5%).

Conclusion

Our results indicated that HLJDD regulated blood circulation by inhibiting platelet aggregation and thrombosis, which might also extensively contribute to the clinical prevention and treatment of cerebrovascular diseases.



中文翻译:

黄连解毒汤在大鼠缺血再灌注脑卒中模型中的抗血栓作用

摘要

语境

黄连解毒汤(HLJJD)具有多种药理活性,如抗炎和抗缺血性脑损伤的神经保护作用。

目标

这项离体研究探讨了其抗血栓形成活性和对血小板聚集的抑制作用。

材料与方法

为了研究 HLJJD离体的抗血栓形成活性,生理盐水或 HLJDD(100、200 和 500 mg/kg)在 Wistar 大鼠中通过灌胃预防性治疗 3 天(n  = 4)。以一过性大脑中动脉梗死(MCAO)或正常大鼠模型(n  =4)为基础,评价正常组和HLJDD亚组在凝血酶原时间、血栓重量、血小板聚集等方面的抗血栓活性,其次是其主要成分的抗血小板聚集(n  = 4)。

结果

MCAO 发病后 24 小时血栓重量显着增加。HLJJD不影响PT的变化,但在降低血栓干重方面显着抑制血栓形成12.5、20.0和20.5%,抑制胶原诱导的血小板聚集25.5、39.0和42.7%,抑制血小板粘附。 17.3、26.2 和 27.3%。HLJJD对胶原诱导的血小板聚集的IC 50值为670 mg/kg。Geniposide 仅促进由胶原蛋白诱导的抗血小板聚集,但不促进 AA 或 ADP。黄芩苷和小檗碱均显示出由所有激活剂诱导的显着抗血小板聚集。黄芩苷的抗血栓活性相对高于黄连素(35.0-47.8% vs. 20.6-33.5%)。

结论

我们的研究结果表明,HLJDD通过抑制血小板聚集和血栓形成来调节血液循环,这也可能广泛地有助于临床防治脑血管疾病。

更新日期:2021-07-01
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