An analytical method was established to identify and quantify hydroxycinnamic acids, such as 1,5-dicaffeoylquinic acid (DCQA) and chicoric acid (CA), in mixtures of Saussurea grandifolia and Taraxacum coreanum (MST) by using reverse-phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Analyses were carried out by using an INNO C18 column with a gradient elution system, and different parameters were used to validate our optimized method. Results demonstrated limits of detection and quantification of 5.46 × 10–3 and 16.54 × 10–3 mg/mL for DCQA and 0.37 × 10–3 and 1.14 × 10–3 mg/mL for CA, respectively. The calibration curves for DCQA and CA showed good linearity over the concentration ranges of 0.025–0.4 and 0.00625–0.1 mg/mL, respectively, and both exhibited r2 = 1.0000. In the accuracy test, high recovery rates were obtained ranging from 101.16–104.18% for DCQA and 97.55–108.49% for CA, while the precision values were ≤ 1.00% for DCQA and ≤ 1.21% for CA. The values obtained from our analyses support the use of this analytical method for the accurate identification and quantification of DCQA and CA from MST. Our methodology could be used further to determine the content of hydroxycinnamic acid derivatives in routine analyses and large-scale extraction processes.

中文翻译：

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优化分析 HPLC-DAD 方法检测雪莲和蒲公英混合物中羟基肉桂酸衍生物

建立了一种分析方法，通过使用反相高性能液体来鉴定和量化大叶雪莲和蒲公英 (MST) 混合物中的羟基肉桂酸，例如 1,5-二咖啡酰奎宁酸 (DCQA) 和菊苣酸 (CA)色谱联用二极管阵列检测器 (HPLC-DAD)。使用带有梯度洗脱系统的 INNO C18 色谱柱进行分析，并使用不同的参数来验证我们优化的方法。结果表明，DCQA 的检测限和定量限分别为 5.46 × 10-3 和 16.54 × 10-3 mg/mL，CA 的检测限和定量限分别为 0.37 × 10-3 和 1.14 × 10-3 mg/mL。DCQA 和 CA 的校准曲线分别在 0.025–0.4 和 0.00625–0.1 mg/mL 的浓度范围内显示出良好的线性，并且均显示 r2 = 1.0000。在精度测试中，获得了高回收率，DCQA 为 101.16-104.18%，CA 为 97.55-108.49%，而 DCQA 的精密度值≤ 1.00%，CA ≤ 1.21%。从我们的分析中获得的值支持使用这种分析方法准确识别和量化来自 MST 的 DCQA 和 CA。我们的方法可进一步用于确定常规分析和大规模提取过程中羟基肉桂酸衍生物的含量。