This work aimed to evaluate the nutrigenomic effect of feeding pike silverside larvae with rotifer versus a formulated microdiet. Survival of larvae was evaluated across the experiment, while growth performance and transcriptional expression profiles in both dietary treatments were compared at the end. Larvae fed with rotifers achieved higher growth and survival, and in accordance, relevant genes associated with growth and development were overexpressed. Moreover, genes associated with digestive enzymes were overexpressed in larvae fed with rotifers, suggesting this to be an average expression profile that may give insight into the digestive status/capacity of pike silverside larvae. On the contrary, the overexpression of genes related to stress, genome instability and epigenetic marks could explain the low larval performance observed in the microdiet fed larvae. These findings point out the relevance of a nutrigenomic approach as an important tool to assess artificial diet performance in fish and the necessity to re-evaluate formulation, type and proportion of the ingredients in the microdiet which may reflect similar expression profiles found in the rotifer group and provide adequate growth in early larval stages of pike silverside. This information contributes to the knowledge of the limitations of microdiets in pike silverside and other fish larvae.

中文翻译：

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使用营养基因组学评估梭鱼幼虫的微量饲料性能

这项工作旨在评估用轮虫与配方微量饲料喂养梭子鱼幼虫的营养基因组学效果。在整个实验中评估幼虫的存活率，同时在最后比较两种饮食处理中的生长性能和转录表达谱。用轮虫喂养的幼虫获得了更高的生长和存活率，相应地，与生长和发育相关的相关基因被过度表达。此外，与消化酶相关的基因在轮虫喂养的幼虫中过度表达，这表明这是一个平均表达谱，可以深入了解梭鱼幼虫的消化状态/能力。相反，压力相关基因的过度表达，基因组不稳定性和表观遗传标记可以解释在微饮食喂养的幼虫中观察到的低幼虫表现。这些发现指出了营养基因组学方法作为评估鱼类人工饲料性能的重要工具的相关性，以及重新评估微量饲料中成分的配方、类型和比例的必要性，这可能反映在轮虫组中发现的相似表达谱并在梭子鱼的早期幼虫阶段提供足够的生长。这些信息有助于了解梭鱼和其他鱼类幼体微量饮食的局限性。微量饲料中成分的类型和比例，这可能反映在轮虫组中发现的相似表达谱，并在梭子鱼的早期幼虫阶段提供足够的生长。这些信息有助于了解梭鱼和其他鱼类幼体微量饮食的局限性。微量饲料中成分的类型和比例，这可能反映在轮虫组中发现的相似表达谱，并在梭子鱼的早期幼虫阶段提供足够的生长。这些信息有助于了解梭鱼和其他鱼类幼体微量饮食的局限性。