Abstract

To determine the effect of growth stages on the essential oil quality for medicinal and industrial prospective, Ocimum sanctum L. was collected from three different sites of north India at the vegetative and full blooming stage. Soils collected from these sites were analyzed and the results reflected that the pH scale ranged from 6.36 to 6.76, electrical conductivity from 0.51 to 0.64 dS m⁻¹, organic carbon from 4.04 to 5.95 %, and water holding capacity from 35.96–59.02 %. GC/MS analysis of the oils resulted in the identification of total 21, 23, 30, 34, 21, and 31 compounds representing 95.73 %, 98.94 %, and 95.10 % of the oil at vegetative and 97.16 %, 99.54 %, and 94.94 % of the oil at the full blooming stage. The effect was most notable in the essential oil composition at all growth stages. β-Elemene (26.52–57.92 %), eugenol (1.22-45.06 %), (E)-β-ocimene (0.01-17.74 %), germacrene D (0.04–17.55 %), (E)-caryophyllene (8.70–39.31 %) and α-copaene (1.24–7.11 %) were the main components of the oils. On the other hand, the predominance of sesquiterpene hydrocarbons (52.30–79.82 %) was higher in both stages of oil composition than followed by others. It was found that during the first harvest, β-elemene (57.92 %) was present as the major compound in the oil while eugenol (45.06 %) was the predominant component in the second harvest.

摘要

为了确定生长阶段对药用和工业前景的精油质量的影响，从印度北部的三个不同地点收集了处于营养和盛开阶段的罗勒。对从这些地点收集的土壤进行了分析，结果表明 pH 值范围为 6.36 至 6.76，电导率范围为 0.51 至 0.64 dS m ^-1，有机碳从 4.04 到 5.95 %，持水能力从 35.96 到 59.02 %。对油进行 GC/MS 分析，共鉴定出 21、23、30、34、21 和 31 种化合物，分别代表植物油的 95.73%、98.94% 和 95.10% 以及 97.16%、99.54.9 和 4% % 盛开阶段的油。在所有生长阶段的精油组合物中，效果最为显着。β-榄香烯 (26.52–57.92 %)、丁香酚 (1.22-45.06 %)、( E )-β-罗勒烯 (0.01-17.74 %)、锗烯 D (0.04–17.55 %)、( E)-石竹烯 (8.70–39.31%) 和 α-可巴烯 (1.24–7.11%) 是油的主要成分。另一方面，在油组成的两个阶段中，倍半萜烃的优势（52.30-79.82%）高于其他阶段。发现在第一次收获期间，β-榄香烯 (57.92%) 作为油中的主要化合物存在，而丁香酚 (45.06%) 是第二次收获中的主要成分。