Abstract—Neutrophil extracellular traps (NET) play an important role in a body’s antimicrobial defense. However, excessive formation leads to various pathological processes in the body. Therefore, a quantitative assessment of the NET content in human whole blood is very important in clinical practice. An approach is proposed allowing the use of polymer microspheres (PMS), the surfaces of which are modified with heparin as sorbents for isolation from whole blood and quantitative determination of NET. To solve this problem, polystyrene microspheres with an average diameter of 140 μm were synthesized, which contain amino groups; covalent immobilization of heparin on their surface was carried out, and a close to linear relationship between the amount of NET and DNA-histone complexes binding to the surface of the synthesized PMS was demonstrated. The data indicate that the approach may be promising both in basic research and clinical practice.

摘要—中性粒细胞胞外陷阱 (NET) 在人体的抗菌防御中发挥重要作用。然而，过度的形成会导致体内的各种病理过程。因此，定量评估人全血中的 NET 含量在临床实践中非常重要。提出了一种允许使用聚合物微球 (PMS) 的方法，其表面用肝素修饰作为吸附剂，用于从全血中分离和定量测定 NET。为了解决这个问题，合成了平均直径为140 μm的聚苯乙烯微球，其中含有氨基；进行了肝素在其表面的共价固定，并证明了 NET 的量与结合到合成 PMS 表面的 DNA-组蛋白复合物之间的接近线性关系。