Abstract—At present, quite a large number of inducers forming neutrophilic extracellular traps (NET) have been described. Mechanical factors (MF) are of particular importance, since they are constantly acting as neutrophils pass through the microcirculatory system. An adequate choice of therapy for various diseases accompanied by excessive NET formation requires assessment of the ability of a patient’s neutrophils to form NET in response to various inducers. Studying the role of MF in neutrophil activation in patients presents certain difficulties associated with introducing expensive and complex recording systems into laboratory practice. A possible alternative to the existing approaches can be joint incubation of whole blood with hemocompatible polymer microspheres (PMS). To develop this approach, polystyrene microspheres containing quaternary ammonium groups were obtained, on the surface of which hemocompatible aggregates consisting of thiooctadecyl poly-N-vinyl-2-pyrrolidone (PMS_nano) were attached. The data obtained indicate that with incubation of blood samples with PMS_nano under gentle stirring, the amount of formed NET is statistically significantly higher than in a stationary incubation mode. This confirms the idea that MF are capable of activating neutrophils and triggering the NET formation process. The proposed approach to assessing the effect of MF on neutrophil activation processes owing to its ease of implementation may find wide application both in biomedical research and clinical laboratories.

摘要——目前，已经描述了相当多的形成嗜中性细胞外陷阱（NET）的诱导剂。机械因素 (MF) 尤其重要，因为它们在中性粒细胞通过微循环系统时不断起作用。对伴有过度 NET 形成的各种疾病的适当治疗选择需要评估患者的中性粒细胞对各种诱导剂形成 NET 的能力。研究 MF 在患者中性粒细胞活化中的作用存在与将昂贵且复杂的记录系统引入实验室实践相关的某些困难。现有方法的一种可能替代方法是将全血与血液相容性聚合物微球 (PMS) 联合培养。为了开发这种方法，_nano ) 已附加。获得的数据表明，在温和搅拌下用 PMS _nano孵育血样，形成的 NET 量在统计上显着高于固定孵育模式。这证实了 MF 能够激活中性粒细胞并触发 NET 形成过程的想法。由于其易于实施，所提议的评估 MF 对中性粒细胞活化过程影响的方法可能会在生物医学研究和临床实验室中得到广泛应用。