ABSTRACT

In the current study,Ferula assa foetida seed oil (FS) (FSEO) was loaded into Solid lipid nanoparticles (SLN) to analyze its apoptotic and anti-angiogenic potential, respectively. The FSEO-loaded SLN (FSEO-SLN) was synthesized and characterized by TEM, DLS, FTIR, and Zeta potential analysis. The cytotoxic, apoptotic, and anti-angiogenic activities of the FSEO-SLN were evaluated by studying the NT2 survival rate, cell cycle phases, and apoptotic gene expression profile (TNF-α, P21, and Cas3). Finally, the impact of FSEO-SLN on the length and number of the CAM blood vessels were analyzed. The FSEO-SLN nanoparticles exhibited a significant cell-growth suppressive impact on the human NT-2 cancer stem cell line. They induced apoptotic death by up-regulating the expression of TNF-α, P21, Cas3 genes. The FSEO-SLN remarkably suppressed angiogenesis in CAM tissue by decreasing the length and number of its blood vessels. Therefore, it has the potential to be studied as an efficient anti-cancer agent.

摘要

在目前的研究中，Ferula assa foetida 将种子油 (FS) (FSEO) 加载到固体脂质纳米颗粒 (SLN) 中，分别分析其凋亡和抗血管生成潜力。合成了负载 FSEO 的 SLN (FSEO-SLN)，并通过 TEM、DLS、FTIR 和 Zeta 电位分析对其进行了表征。通过研究 NT2 存活率、细胞周期阶段和凋亡基因表达谱（TNF-α、P21 和 Cas3）来评估 FSEO-SLN 的细胞毒性、凋亡和抗血管生成活性。最后分析了FSEO-SLN对CAM血管长度和数量的影响。FSEO-SLN 纳米粒子对人类 NT-2 癌症干细胞系表现出显着的细胞生长抑制作用。他们通过上调 TNF-α、P21、Cas3 基因的表达来诱导细胞凋亡。FSEO-SLN 通过减少其血管的长度和数量来显着抑制 CAM 组织中的血管生成。因此，它具有作为一种有效的抗癌剂进行研究的潜力。