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Preharvest application of melatonin induces anthocyanin accumulation and related gene upregulation in red pear (Pyrus ussuriensis)
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2021-06-29 , DOI: 10.1016/s2095-3119(20)63312-3
Hui-li SUN , Xin-yue WANG , Ye SHANG , Xiao-qian WANG , Guo-dong DU , De-guo LÜ

Anthocyanins are important components in the peel of red pears and contribute to the appearance of the fruit. Melatonin application is known to affect anthocyanin biosynthesis, but the effect of preharvest melatonin application on fruit coloration remains largely unknown. The objective of this study was to determine the effects of preharvest melatonin application on pigmentation, phenolic compounds, and the expression of related genes in Nanhong pear (Pyrus ussuriensis). The applications were performed during the pre-color-change period by spraying 50 or 200 µmol L−1 of melatonin on fruits. We found that treatment with melatonin had a significant effect on color development. The concentrations of anthocyanins and flavonols were enhanced by melatonin treatment, whereas hydroxycinnamate and flavanol concentrations were reduced. Quantitative real-time PCR analyses indicated that the transcription levels for most anthocyanin biosynthetic genes and anthocyanin-related transcription factors were induced by melatonin. Melatonin application also stimulated the expression of melatonin biosynthesis-related genes and consequently caused an increase in endogenous melatonin concentration. These results provide insights into melatonin-induced fruit coloration and will facilitate the application of exogenous melatonin in agriculture.



中文翻译:

褪黑激素的采前施用诱导红梨(Pyrus ussuriensis )花青素积累和相关基因上调

花青素是红梨果皮中的重要成分,有助于果实的外观。已知应用褪黑激素会影响花青素的生物合成,但收获前应用褪黑激素对果实着色的影响仍然未知。本研究的目的是确定采前施用褪黑激素对南红梨(Pyrus ussuriensis)色素沉着、酚类化合物和相关基因表达的影响应用在预变色期间通过喷洒 50 或 200 µmol L -1褪黑激素对水果的影响。我们发现用褪黑激素处理对颜色发展有显着影响。褪黑激素处理提高了花青素和黄酮醇的浓度,而羟基肉桂酸酯和黄烷醇的浓度降低了。定量实时 PCR 分析表明,大多数花青素生物合成基因和花青素相关转录因子的转录水平是由褪黑激素诱导的。褪黑激素的应用还刺激了褪黑激素生物合成相关基因的表达,从而导致内源性褪黑激素浓度增加。这些结果提供了对褪黑激素诱导的果实着色的见解,并将促进外源褪黑激素在农业中的应用。

更新日期:2021-06-30
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