Plant growth-promoting bacteria (PGPB), possess multifarious beneficial traits and facilitate plant growth by both direct and indirect mechanisms under hostile conditions. The objective of the current study was to evaluate the potential application of 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) producing plant-growth promoting endophyte bacterium (PGPEB) Kocuria rhizophila strain 14ASP (National Center for Biotechnology Information; NCBI) Accession Number: LFIY00000000.1) heavy metals and salt resistance tolerance under hostile environment. The in vitro study was conducted to evaluate plant growth promotion characteristics, heavy metal and salt resistance of the K. rhizophila under normal and stressful environments. The strain 14ASP revealed the plant-growth promoting and of AAC deaminase activities under abiotic stress environment. K. rhizophila showed significant tolerance against different heavy metals on LB (Luria Ber-tani) agar plates and broth medium supplemented with cadmium (Cd), (50 mg L⁻¹), cooper (Cu), (50 mg L⁻¹), nickel (Ni), (50 mg L⁻¹), lead (Pb), (200 mg L⁻¹) and chromium (Cr) (500 mg L⁻¹), respectively. It also exhibited population density (OD = 0.31–0.45) at different concentrations of sodium chloride (NaCl) (0% 5%, 10%, and 15% w/v) in LB medium. The draft genome of strain 14ASP is 2.6 Mb and was assembled into 183 contigs with 2689,1 bp and a G + C content of 70% and 1,882,646 bp, encoded 2409 protein, 8 rRNAs and 46 tRNAs. Genome analysis identified genes, involved in hydrocarbon metabolism, heavy metal tolerance, biofilm formation, Indole Acetic Acid (IAA) and siderophore biosynthesis. All these properties were confirmed by in silico and draft genome analysis.

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中文翻译：

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农业和环境管理中的生物技术方法 - 细菌 Kocuria rhizophila 14ASP 作为重金属和耐盐植物生长促进菌株

植物生长促进细菌 (PGPB) 具有多种有益特性，可在恶劣条件下通过直接和间接机制促进植物生长。本研究的目的是评估产生 1-氨基环丙烷-1-羧酸脱氨酶（ACC 脱氨酶）的植物生长促进内生菌（PGPEB）Kocuria rhizophila菌株 14ASP（国家生物技术信息中心；NCBI）的潜在应用: LFIY00000000.1) 重金属和恶劣环境下的耐盐性。进行体外研究以评估K. rhizophila 的植物生长促进特性、重金属和耐盐性在正常和压力环境下。菌株 14ASP 揭示了非生物胁迫环境下植物生长促进和 AAC 脱氨酶活性。K. rhizophila在 LB (Luria Ber-tani) 琼脂平板和补充有镉 (Cd)、(50 mg L ^-1 )、铜 (Cu)、(50 mg L ^-1 ) 的肉汤培养基上对不同的重金属表现出显着耐受性、镍（Ni）、（50 mg L ^-1）、铅（Pb）、（200 mg L ^-1）和铬（Cr）（500 mg L ^-1）。它还在不同浓度的氯化钠 (NaCl) (0% 5%、10% 和 15% w / v ) 下表现出种群密度 (OD = 0.31–0.45)) 在 LB 培养基中。菌株 14ASP 的基因组草图为 2.6 Mb，组装成 183 个重叠群，2689.1 bp，G + C 含量为 70% 和 1,882,646 bp，编码 2409 个蛋白质、8 个 rRNA 和 46 个 tRNA。基因组分析确定了涉及碳氢化合物代谢、重金属耐受性、生物膜形成、吲哚乙酸 (IAA) 和铁载体生物合成的基因。所有这些特性都通过计算机模拟和基因组草图分析得到证实。

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