Aim It’s been reported that pro-inflammatory cytokines are elevated in patients with diabetic retinopathy (DR); this may contribute to the pathophysiology of the disease. The aim of this study is to measure the concentration of various inflammatory cytokines from the main CD4+ T helper inflammatory responses in blood serum from Mexican patients with DR in different stages using cytometric bead array (CBA) technology and correlate them with the presence and severity of DR in order to find possible DR biomarkers that serve as diagnostic or therapeutic predictors. Methods 64 subjects were included in the study, 16 in the control group, 16 in the type 2 diabetes mellitus no DR (NDR) group, 16 in the non-proliferative DR (NPDR) group and 16 in the proliferative DR (PDR) group. Cytokine concentrations of interleukin (IL) 1ß, IL‐2, IL‐4, IL‐6, IL‐8, IL‐10, IL‐12, IL‐17A, tumour necrosis factor alpha (TNFα) and interferon-gamma in serum samples were measured using Human Inflammatory and TH1/TH2/TH17 CBA Kit. Results IL-6, IL-12, IL-17a and TNFα were significantly higher in the patients with DR compared with the control group. The PDR group showed a slightly lower concentration of serum cytokines IL-6, IL-12 and IL-17a. TNFα showed a higher concentration compared with healthy controls, NDR and NPDR subjects. We also found a positive statistical correlation between the presence and severity of DR with the clinical parameters haemoglobin A1c, body mass index and serum creatinine and the concentration of serum cytokines IL-6 and TNFα. Conclusion Our findings suggest that patients with diabetes and DR have a stronger chronic inflammatory profile compared with non-diabetic subjects. There are no data in this work.

中文翻译：

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促炎细胞因子谱存在于墨西哥患有不同阶段继发于 2 型糖尿病的糖尿病视网膜病变患者的血清中

目的 据报道，糖尿病视网膜病变（DR）患者的促炎细胞因子升高；这可能有助于该疾病的病理生理学。本研究的目的是使用细胞计数珠阵列 (CBA) 技术测量来自墨西哥不同阶段 DR 患者血清中主要 CD4+ T 辅助炎症反应的各种炎性细胞因子的浓度，并将它们与存在和严重程度相关联。 DR 以找到可能的 DR 生物标志物作为诊断或治疗预测因子。方法 64 名受试者入组，对照组 16 名，2 型糖尿病无 DR（NDR）组 16 名，非增殖性 DR（NPDR）组 16 名，增殖性 DR（PDR）组 16 名. 白细胞介素 (IL) 1ß、IL-2、IL-4、IL-6、IL-8、使用 Human Inflammatory 和 TH1/TH2/TH17 CBA 试剂盒测量血清样品中的 IL-10、IL-12、IL-17A、肿瘤坏死因子 α (TNFα) 和干扰素-γ。结果与对照组相比，DR患者的IL-6、IL-12、IL-17a和TNFα显着升高。PDR组的血清细胞因子IL-6、IL-12和IL-17a浓度略低。与健康对照、NDR 和 NPDR 受试者相比，TNFα 显示出更高的浓度。我们还发现 DR 的存在和严重程度与临床参数血红蛋白 A1c、体重指数和血清肌酐以及血清细胞因子 IL-6 和 TNFα 的浓度呈正相关。结论 我们的研究结果表明，与非糖尿病受试者相比，糖尿病和 DR 患者具有更强的慢性炎症特征。