Spodoptera frugiperda is an important pest of several crops. Use of chemical insecticides in insect control has not been successful. In the present study, bio-nano formulation of bimetallic nanoparticles were synthesized using fungal metabolites from Metarhizium anisopliae and tested for insecticidal activity. Metarhizium anisopliae metabolites was synthesized along with bimetallic nanoparticles at different volumes for bioassay studies. Bimetallic nanoparticles showed colour change from light greenish to white. Synthesis of bimetallic NPs was confirmed by UV–vis spectrophotometer, with absorption peak at 387 nm. Functional groups in the synthesized BMNPs were analyzed by FTIR, which showed the presence of functional amines, carboxylic acids, alkenes, alkyl halides, phosphine oxides, anhydrides, phosphines, sulfonates, acid chlorides and alkynes. XRD analysis confirms the crystalline nature of nanoparticles. SEM analysis exhibits, the nanoparticles to be spherical in shape with size ranging 9.50 nm. EDaX analysis confirmed the elemental composition of synthesized NPs, while DLS for size distribution ranging at 80 to 137 nm. The larvicidal activity of monometallic and mycogenic bimetallic nanoparticles were tested on third instar S. frugiperda larvae. The highest mortality was observed in BMNPs at a concentration of 100 μg/ml after 48 h exposure. Pupicidal and antifeedant activity was also observed in the tested BMNPs. Detoxification enzymes studies of BMNPs showed considerable lowering these enzyme which may result in toxic manifestation in the insect. These results strongly suggest that, bimetallic nanoparticles synthesized using M. anisopilae can be used for protecting the crops against S. furgiperda.

草地夜蛾是多种作物的重要害虫。在昆虫控制中使用化学杀虫剂尚未成功。在本研究中，使用绿僵菌的真菌代谢物合成了双金属纳米粒子的生物纳米制剂，并测试了杀虫活性。绿僵菌代谢物与不同体积的双金属纳米粒子一起合成，用于生物测定研究。双金属纳米粒子的颜色从浅绿色变为白色。双金属纳米颗粒的合成由紫外可见分光光度计证实，吸收峰位于 387 nm。合成的 BMNPs 中的官能团通过 FTIR 分析，表明存在官能胺、羧酸、烯烃、卤代烷、氧化膦、酸酐、膦、磺酸盐、酰氯和炔烃。XRD 分析证实了纳米颗粒的结晶性质。SEM 分析表明，纳米颗粒为球形，尺寸范围为 9.50 nm。EDaX 分析证实了合成 NPs 的元素组成，而 DLS 的尺寸分布范围为 80 至 137 nm。S. frugiperda幼虫。在暴露 48 小时后，浓度为 100 μg/ml 的 BMNPs 的死亡率最高。在测试的 BMNP 中也观察到了杀瞳和拒食活性。BMNPs 的解毒酶研究表明这些酶显着降低，这可能导致昆虫出现毒性。这些结果有力地表明，使用M. anisopilae合成的双金属纳米粒子可用于保护作物免受S. furgiperda 的侵害。