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Phytotoxicity, cytotoxicity and chemical composition of Spondias mombin Linn. Stem bark
Clinical Phytoscience Pub Date : 2021-06-30 , DOI: 10.1186/s40816-021-00297-x
Kissinger Obaogie Orumwensodia , Patrick Ojeifo Uadia , Muhammed Iqbal Choudhary

Spondias mombin Linn. is a tropical climate plant with wide applications in ethnomedicinal practice. This study evaluates the phytotoxicity, cytotoxicity and chemical composition of the plant’s stem bark. Dried stem bark sample of Spondias mombin Linn. was subjected to exhaustive extraction and partitioned into sub-fractions (hexane-ethylacetate, ethylacetate, ethylacetate-methanol and methanol) by graded polarity technique. The phytotoxicity and cytotoxicity indices of the crude hydro-ethanol extract and fractions were evaluated using Lemna minor and brine shrimp lethality assays, respectively, while chemical composition of the oily hexane:ethylacetate fraction was determined by gas chromatography-mass spectroscopy (GC-MS) technique. Phytotoxicity was dose-dependent which ranged from low (crude plant extract), moderate (hexane-ethylacetate and methanol fractions), high (ethylaacetate-methanol fraction) to significant toxicity (ethylacetate fraction) at the highest dose. However, for brine shrimp lethality assay only hexane-ethylacetate (LD50: 284.02 μg/mL) and ethylacetate (LD50: 210.24 μg/mL) fractions were cytotoxic at the highest dose. The GC-MS profile of the oily hexane:ethylacetate fraction identified sixty-eight compounds comprising hydrocarbons, fatty acids, alcohols, steroids, nitrogen and fluoride-containing compounds, terpenes and esters. This study concludes that fractions of Spondias mombin Lin. could be potentially toxic. While its phytotoxic potential can be useful in the agrochemical industry for the production of natural herbicides, its cytotoxic property can be cautiously harnessed for ethnomedicinal purposes.

中文翻译:

Spondias mombin Linn 的植物毒性、细胞毒性和化学成分。茎皮

Spondias mombin Linn。是一种热带气候植物,在民族医学实践中具有广泛的应用。本研究评估了植物茎皮的植物毒性、细胞毒性和化学成分。Spondias mombin Linn 的干茎皮样本。进行彻底萃取并通过梯度极性技术分配为亚级分(己烷-乙酸乙酯、乙酸乙酯、乙酸乙酯-甲醇和甲醇)。粗水乙醇提取物和馏分的植物毒性和细胞毒性指数分别使用 Lemna minor 和盐水虾致死率测定进行评估,而油性己烷:乙酸乙酯馏分的化学成分通过气相色谱-质谱法 (GC-MS) 确定技术。植物毒性是剂量依赖性的,范围从低(粗植物提取物)到 在最高剂量时,中度(己烷-乙酸乙酯和甲醇部分)、高(乙酸乙酯-甲醇部分)到显着毒性(乙酸乙酯部分)。然而,对于盐水虾致死率测定,只有己烷-乙酸乙酯(LD50:284.02 μg/mL)和乙酸乙酯(LD50:210.24 μg/mL)组分在最高剂量下具有细胞毒性。油性己烷:乙酸乙酯馏分的 GC-MS 分析确定了 68 种化合物,包括烃、脂肪酸、醇、类固醇、含氮和氟化物的化合物、萜烯和酯。这项研究的结论是,Spondias mombin Lin 的分数。可能具有潜在毒性。虽然其潜在的植物毒性可用于农业化学工业生产天然除草剂,但其细胞毒性可谨慎用于民族医学目的。最高剂量时从高(乙酸乙酯-甲醇部分)到显着毒性(乙酸乙酯部分)。然而,对于盐水虾致死率测定,只有己烷-乙酸乙酯(LD50:284.02 μg/mL)和乙酸乙酯(LD50:210.24 μg/mL)组分在最高剂量下具有细胞毒性。油性己烷:乙酸乙酯馏分的 GC-MS 分析确定了 68 种化合物,包括烃、脂肪酸、醇、类固醇、含氮和氟化物的化合物、萜烯和酯。这项研究的结论是,Spondias mombin Lin 的分数。可能具有潜在毒性。虽然其潜在的植物毒性可用于农业化学工业生产天然除草剂,但其细胞毒性可谨慎用于民族医学目的。最高剂量时从高(乙酸乙酯-甲醇部分)到显着毒性(乙酸乙酯部分)。然而,对于盐水虾致死率测定,只有己烷-乙酸乙酯(LD50:284.02 μg/mL)和乙酸乙酯(LD50:210.24 μg/mL)组分在最高剂量下具有细胞毒性。油性己烷:乙酸乙酯馏分的 GC-MS 分析确定了 68 种化合物,包括烃、脂肪酸、醇、类固醇、含氮和氟化物的化合物、萜烯和酯。这项研究的结论是,Spondias mombin Lin 的分数。可能具有潜在毒性。虽然其潜在的植物毒性可用于农业化学工业生产天然除草剂,但其细胞毒性可谨慎用于民族医学目的。
更新日期:2021-06-30
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