Macrophages have a variety of functions, such as secreting cytokines, phagocytosis, et al. Tripartite motif containing 59 (TRIM59) protein is highly expressed in tumor cells. It can regulate proliferation of tumor cells and promote tumor progression. Recent studies shown that the expression of TRIM59 was different in macrophages when stimulated by different stimuli, however, the effects of TRIM59 on macrophage gene expression profiles and functions are still unknown. In our study, we constructed RAW264.7 macrophages with high and low expression of TRIM59, and used next generation sequencing to explore the effects of TRIM59 on macrophage gene expression profiles. Results showed that TRIM59 affected an abundant number of genes, and may affect phagocytosis and cell cycles. We also examined the expression of surface molecules, secretion of cytokines, phagocytosis, proliferation, and apoptosis of macrophages, and confirmed that TRIM59 increased the expression of FcγRs CD16/32, CD64 and the secretion of TNF-α and IL-10, promoted phagocytosis and proliferation of RAW264.7 cells, inhibited the expression of complement receptor CD11b and antigen presentation related receptors (MHCII, CD80), but TRIM59 had no significant effect on apoptosis. Our study explored the effect of TRIM59 on the gene expression and function of macrophages comprehensively.

巨噬细胞具有多种功能，如分泌细胞因子、吞噬作用等。含有 59 (TRIM59) 蛋白的三方基序在肿瘤细胞中高度表达。可调节肿瘤细胞增殖，促进肿瘤进展。最近的研究表明，TRIM59在受到不同刺激时在巨噬细胞中的表达是不同的，然而，TRIM59对巨噬细胞基因表达谱和功能的影响仍然未知。在我们的研究中，我们构建了TRIM59高表达和低表达的RAW264.7巨噬细胞，并使用下一代测序来探索TRIM59对巨噬细胞基因表达谱的影响。结果显示TRIM59影响大量基因，并可能影响吞噬作用和细胞周期。我们还检查了表面分子的表达、细胞因子的分泌、巨噬细胞的吞噬、增殖和凋亡，证实TRIM59增加FcγRs CD16/32、CD64的表达和TNF-α和IL-10的分泌，促进RAW264.7细胞的吞噬和增殖，抑制补体的表达受体CD11b和抗原呈递相关受体（MHCII，CD80），但TRIM59对细胞凋亡没有显着影响。我们的研究全面探讨了TRIM59对巨噬细胞基因表达和功能的影响。但TRIM59对细胞凋亡没有显着影响。我们的研究全面探讨了TRIM59对巨噬细胞基因表达和功能的影响。但TRIM59对细胞凋亡没有显着影响。我们的研究全面探讨了TRIM59对巨噬细胞基因表达和功能的影响。