BCL2 family proteins are important regulators of mitochondrial outer membrane permeabilization (MOMP). In recent years, BCL2 family proteins have also been linked to the regulation of mitochondrial bioenergetics and dynamics. Given their overexpression in breast cancer cells, we sought to explore whether two key members of this family, BCL2 and BCL(X)L impacted on mitochondrial fusion/fission processes. By employing a single cell imaging and RNA sequencing we found that overexpression of BCL2 or BCL(X)L increases mitochondrial dynamics and alters the expression profile of genes involved in this process. Collectively, our data show that overexpression of BCL2 proteins regulates mitochondrial dynamics in breast cancer tumor cells.

BCL2 家族蛋白是线粒体外膜透化 (MOMP) 的重要调节因子。近年来，BCL2 家族蛋白也与线粒体生物能量学和动力学的调节有关。鉴于它们在乳腺癌细胞中的过度表达，我们试图探索该家族的两个关键成员 BCL2 和 BCL( X )L 是否影响线粒体融合/裂变过程。通过使用单细胞成像和 RNA 测序，我们发现 BCL2 或 BCL( X )L 的过度表达会增加线粒体动力学并改变参与该过程的基因的表达谱。总的来说，我们的数据表明 BCL2 蛋白的过表达调节了乳腺癌肿瘤细胞中的线粒体动力学。