Abstract

The Drosophila TTK protein is involved in the processes of cell differentiation and is represented by two isoforms, TTK69 and TTK88, which have a common N-terminal BTB domain and different C-terminal sequences. Earlier, it was shown that TTK69 represses the activity of enhancers and promoters by recruiting a conserved among higher eukaryotes NURD complex to chromatin. The Mep-1 protein was found in the NURD-complex of Drosophila, and this protein can interact with the C-terminal region of TTK69. In the present study, using the yeast two-hybrid system, we mapped the interacting regions of the TTK and Mep-1 proteins. We identified regions in the unique C-terminal regions of TTK isoforms that can interact simultaneously with two regions of the Mep-1 protein. The results show that, despite the low homology of the C-terminal regions, the TTK isoform retains the ability to interact with two conserved regions of the Mep-1 protein, which suggests the functional significance of this interaction.

中文翻译：

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TTK 同种型与黑腹果蝇 Mep-1 蛋白的两个区域相互作用

摘要

在果蝇TTK蛋白参与细胞分化的方法和通过两个同种型，和TTK69 TTK88，其具有共同的N端结构域BTB和不同的C端序列表示。早些时候，已经表明 TTK69 通过将高等真核生物中保守的 NURD 复合物募集到染色质来抑制增强子和启动子的活性。在果蝇的 NURD 复合体中发现了 Mep-1 蛋白，并且该蛋白质可以与 TTK69 的 C 端区域相互作用。在本研究中，我们使用酵母双杂交系统绘制了 TTK 和 Mep-1 蛋白的相互作用区域。我们在 TTK 同种型的独特 C 端区域中确定了可以与 Mep-1 蛋白的两个区域同时相互作用的区域。结果表明，尽管 C 端区域的同源性较低，但 TTK 同种型保留了与 Mep-1 蛋白的两个保守区域相互作用的能力，这表明这种相互作用的功能意义。