Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients,Doklady Biochemistry and Biophysics

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Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients
Doklady Biochemistry and Biophysics ( IF 0.8 ) Pub Date : 2021-06-29 , DOI: 10.1134/s1607672921030054
E N Larina ₁ , V S Karasev ₁ , M V Shpilevaya ₁ , T K Aliev ₁ , O P Bochkova ₂ , A E Karamova ₃ , D S Balabashin ₁ , D G Deryabin ₃ , T V Bobik ₁ , I V Smirnov ₁ , A A Kubanov ₃ , S M Staroverov ₂ , A G Gabibov ₁ , M P Kirpichnikov _{1,

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Affiliation

Abstract

Using the recombinant second fragment of the extracellular domain (EC2) of human desmoglein type 3 (Dsg3) as an affinity ligand, an immunosorbent was obtained that selectively binds autoreactive antibodies to this domain from the immune sera of patients with pemphigus. The EC2 protein was obtained in the form of a fusion protein with the Fc-fragment of human IgG1. The production was carried out in CHO cells using the method of transient expression.

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