Abstract

In recent years, diabetes is one of the diseases that cause the most death and disability. It is a global public health problem. Diabetes is mainly due to metabolic disorders caused by insufficient insulin or high blood sugar in the body. Concentration range (4.4–6.6 mM). Therefore, the development of fast and reliable blood glucose sensors has been receiving widespread attention in the diagnosis of diabetes. This article is based on the nano-conjugate electrochemical aptamer sensor, and its performance is studied by detecting dopamine, using the affinity of the nucleic acid aptamer and the target to be stronger than the principle of base force between the nucleic acid aptamer and the complementary strand, and Combined with blood glucose meter to detect dopamine. The results showed that the incubation time of dopamine and the catalytic time of invertase were 30 min and 120 min respectively; the linear detection range of dopamine was 0.45–10 mmol/L, the correlation coefficient was 0.997, and the detection limit was 0.45 mmol/L; The chemical aptamer sensor can maintain the bioelectrocatalytic activity of glucose oxidation, and the electrode detection of glucose shows good electrochemical performance, with high sensitivity, good stability, strong anti-interference ability and other advantages, which can meet the requirements of noninvasive blood glucose detection. Need, has great application prospects in the noninvasive detection of blood glucose.

摘要

近年来，糖尿病是导致死亡和致残最多的疾病之一。这是一个全球性的公共卫生问题。糖尿病主要是由于体内胰岛素不足或高血糖引起的代谢紊乱。浓度范围 (4.4–6.6 mM)。因此，开发快速可靠的血糖传感器在糖尿病诊断中受到广泛关注。本文基于纳米共轭电化学适体传感器，通过检测多巴胺来研究其性能，利用核酸适体与靶标的亲和力强于核酸适体与互补体之间的碱基力原理。链，并结合血糖仪检测多巴胺。结果表明，多巴胺的孵育时间和转化酶的催化时间分别为30 min和120 min；多巴胺的线性检测范围为0.45～10 mmol/L，相关系数为0.997，检出限为0.45 mmol/L；化学适体传感器可保持葡萄糖氧化的生物电催化活性，葡萄糖的电极检测显示出良好的电化学性能，具有灵敏度高、稳定性好、抗干扰能力强等优点，可满足无创血糖检测的要求. 需要，在血糖的无创检测方面具有很大的应用前景。检出限为0.45 mmol/L；化学适体传感器可保持葡萄糖氧化的生物电催化活性，葡萄糖的电极检测显示出良好的电化学性能，具有灵敏度高、稳定性好、抗干扰能力强等优点，可满足无创血糖检测的要求. 需要，在血糖的无创检测方面具有很大的应用前景。检出限为0.45 mmol/L；化学适体传感器可保持葡萄糖氧化的生物电催化活性，葡萄糖的电极检测显示出良好的电化学性能，具有灵敏度高、稳定性好、抗干扰能力强等优点，可满足无创血糖检测的要求. 需要，在血糖的无创检测方面具有很大的应用前景。