In the present study, silver, cadmium, iron and copper nanoparticles were biosynthesized by Salmonella typhimurium, Staphylococcus aureus, Candida albicans and Fusarium avenaceum strains. Analysis of the synthesized nanoparticles using the dynamic light scattering (DLS) revealed that the smallest nanoparticles were obtained by Salmonella typhimurium. The formation of the synthesized bio-AgNPs was confirmed by UV–Vis spectroscopy. The Ag, Cd, Fe and Cu nanoparticles produced by Salmonella typhimurium were separately immobilized on the chitosan (CS) framework by the impregnation method. The prepared CS-bio-nanocomposites were characterized by X-ray diffraction (XRD). The prepared compounds were investigated against three cancer cells (MCF-7, HeLa and HCT-1l6) for anticancer activity as well as against different microbial strains as antimicrobial activity. CS-bioAgNP composite clearly showed the greatest cytotoxicity against different cancer cell lines. Also, the CS-bioAgNP composite resulted in the highest inhibitory effect against different microbial growth strains using disk diffusion and kill time methods when compared with chitosan and other CS-bioNPs composites. Therefore, the present study provides evidence of novel antimicrobial and potent cytotoxicity characteristics of CS-bio-nanocomposite material that can be effectively utilized in the medical field.

在本研究中，银、镉、铁和铜纳米粒子由鼠伤寒沙门氏菌、金黄色葡萄球菌、白色念珠菌和 镰刀菌菌株生物合成。使用动态光散射 (DLS) 分析合成的纳米颗粒表明，最小的纳米颗粒是由鼠伤寒沙门氏菌获得的。UV-Vis光谱证实了合成的生物AgNPs的形成。鼠伤寒沙门氏菌产生的银、镉、铁和铜纳米颗粒通过浸渍方法分别固定在壳聚糖（CS）框架上。通过X射线衍射（XRD）表征制备的CS-生物纳米复合材料。研究了制备的化合物针对三种癌细胞（MCF-7、HeLa 和 HCT-1l6）的抗癌活性以及针对不同微生物菌株的抗微生物活性。CS-bioAgNP 复合物清楚地显示出对不同癌细胞系的最大细胞毒性。此外，与壳聚糖和其他 CS-bioNPs 复合材料相比，CS-bioAgNP 复合材料使用圆盘扩散和杀灭时间方法对不同的微生物生长菌株产生了最高的抑制作用。所以，