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Epidemiologic and molecular characterization of β-lactamase-producing multidrug-resistant uropathogenic Escherichia coli isolated from asymptomatic hospitalized patients
International Microbiology ( IF 3.1 ) Pub Date : 2021-06-30 , DOI: 10.1007/s10123-021-00187-9
Arunita Ghosh 1 , Biplab Ghosh 1 , Mandira Mukherjee 1
Affiliation  

Uropathogenic Escherichia coli (UPECs) are the predominant cause of asymptomatic bacteriuria (ABU) and symptomatic UTI. In this study, multidrug-resistant (MDR) ABU-UPECs from hospitalized patients of Kolkata, India, were characterized with respect to their ESBL phenotype, acquisition of β-lactamase genes, mobile genetic elements (MGEs), phylotype property, ERIC-PCR profile, sequence types (STs), clonal complexes (CCs) and evolutionary and quantitative relationships and compared to the symptomatic ones to understand their epidemiology and evolutionary origin. Statistically significant incidence of ESBL producers, β-lactamase genes, MGEs and novel phylotype property (NPP) among ABU-UPECs similar to the symptomatic ones indicated the probable incidence of chromosomal plasticity on resistance gene acquisition through MGEs due to indiscriminate drug usage. ERIC-PCR typing and MLST analysis showed clonal heterogeneity and predominance of ST940 (CC448) among asymptomatic isolates akin to symptomatic ones along with the evidence of zoonotic transmissions. Minimum spanning tree analysis showed a close association between ABU-UPEC with known and unidentified STs having NPPs with isolates that belonged to phylogroups clade I, D, and B2. This is the first study that reported the occurrence of MGEs and NPPs among ABU-UPECs with the predominance of ESBL production which displayed the deleterious effect of MDR among this pathogen demanding alternative therapeutic interventions. Moreover, this study for the first time attempted to introduce a new approach to ascertain the phylotype property of unassigned UPECs. Withal, increased recognition, proper understanding and characterization of ABU-UPECs with the implementation of appropriate therapeutic measures against them when necessary are the need of the era which otherwise might lead to serious complications in the vulnerable population.



中文翻译:

从无症状住院患者中分离出的产生 β-内酰胺酶的耐多药尿路致病性大肠杆菌的流行病学和分子特征

尿路致病性大肠杆菌(UPEC) 是无症状菌尿 (ABU) 和有症状 UTI 的主要原因。在这项研究中,对来自印度加尔各答住院患者的耐多药 (MDR) ABU-UPEC 的 ESBL 表型、β-内酰胺酶基因的获得、移动遗传元件 (MGE)、系统发育特性、ERIC-PCR 进行了表征概况、序列类型 (STs)、克隆复合物 (CCs) 以及进化和数量关系,并与有症状的关系进行比较,以了解它们的流行病学和进化起源。与症状相似的 ABU-UPEC 中 ESBL 生产者、β-内酰胺酶基因、MGE 和新系统型特性 (NPP) 的统计显着发生率表明,由于滥用药物,通过 MGE 获得抗性基因的染色体可塑性可能发生。ERIC-PCR 分型和 MLST 分析显示 ST940 (CC448) 在类似于有症状的无症状分离株中具有克隆异质性和优势,以及人畜共患病传播的证据。最小生成树分析表明,ABU-UPEC 与已知和未鉴定的 ST 之间存在密切关联,这些 ST 具有 NPP 和属于进化枝 I、D 和 B2 的分离株。这是第一项报告在 ABU-UPECs 中发生 MGEs 和 NPPs 的研究,其中 ESBL 生产占主导地位,这表明 MDR 在这种需要替代治疗干预的病原体中的有害影响。此外,本研究首次尝试引入一种新方法来确定未分配的 UPEC 的系统发育特性。随着认识的增加,

更新日期:2021-06-30
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