Mosquito responds to various insecticides in the presence of environmental factors which is very important to consider in vector management as these factors affect both mosquito behavior and efficacy of insecticides. The current study aims to examine the impact of high temperatures and Bti insecticide on the biochemical profile of Aedes aegypti and Aedes albopictus mosquitoes. Late third instar larvae of Aedes aegypti and Aedes albopictus were exposed to varying temperatures (39 °C and 40 °C) and Bti concentrations (1 ppm and 1.5 ppm). After exposure, detoxifying enzymes such as Esterase (α and β), Glutathione S-transferase (GST), Cytochrome P450 monooxygenase and total protein level were analyzed. It was observed that both temperature and Bti significantly influenced the level of protein and enzyme activities in both mosquito species. Aedes aegypti and Aedes albopictus exhibited a significant increase in total protein in response to temperature exposure at 40 °C and a decline of total protein in response to Bti exposure. Thermally pre-exposed (40 °C) larvae exhibited an increase in total protein content after Bti exposure. The activity of esterase decreased significantly in thermally exposed mosquitoes while Bti exposure increased the level of esterase significantly. The result of the current study also showed a significant increase in GSTs and monooxygenase after exposure to temperature and Bti in both mosquitoes. Thermally pre-exposed larvae of both mosquito species exhibited increased activities of GSTs and monooxygenase in response to Bti exposure. A significant interaction between temperature and Bti for activities of GSTs and monooxygenase was also observed. Current results showed most of the enzymes were highly expressed in Ae. aegypti than Ae. albopictus. Our results suggest that consideration of environmental factors like temperature is crucial while evaluating the efficacy of Bti that may help to make accurate prediction of Bti effect on Aedes mosquitoes.

蚊子在存在环境因素的情况下对各种杀虫剂有反应，这在媒介管理中非常重要，因为这些因素会影响蚊子的行为和杀虫剂的功效。目前的研究旨在检查高温和Bti杀虫剂对埃及伊蚊和白纹伊蚊的生化特征的影响。埃及伊蚊和白纹伊蚊的晚三龄幼虫暴露于不同温度（39°C 和 40°C）和Bti浓度（1 ppm 和 1.5 ppm）。暴露后，分析解毒酶如酯酶（α和β）、谷胱甘肽S-转移酶（GST）、细胞色素P450单加氧酶和总蛋白水平。据观察，温度和Bti 都显着影响了两种蚊子的蛋白质和酶活性水平。Aedes aegypti和Aedes albopictus表现出响应于 40 °C 温度暴露的总蛋白显着增加和响应Bti暴露的总蛋白下降。热预暴露 (40 °C) 幼虫在Bti后表现出总蛋白质含量的增加接触。热暴露蚊子的酯酶活性显着降低，而Bti暴露显着增加酯酶水平。当前研究的结果还显示，在两种蚊子中，暴露于温度和Bti后，GST 和单加氧酶显着增加。两种蚊子的热预暴露幼虫都表现出 GST 和单加氧酶的活性增加，以响应Bti暴露。还观察到温度和Bti之间对 GST 和单加氧酶活性的显着相互作用。目前的结果表明，大多数酶在Ae中高度表达。埃及比Ae。白纹象. 我们的结果表明，在评估Bti的功效时，考虑温度等环境因素至关重要，这可能有助于准确预测Bti对伊蚊的影响。