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Newcastle disease virus V protein interacts with hnRNP H1 to promote viral replication
Veterinary Microbiology ( IF 3.3 ) Pub Date : 2021-06-30 , DOI: 10.1016/j.vetmic.2021.109093
Lina Tong 1 , Zhili Chu 2 , Xiaolong Gao 3 , Mengqing Yang 4 , Fathalrhman Eisa A Adam 4 , Daguia Wenam Prince Theodore 4 , Haijin Liu 4 , Xinglong Wang 4 , Sa Xiao 4 , Zengqi Yang 4
Affiliation  

The interactions between host cellular proteins and viral proteins are important for successful infection by viruses. Previous studies from our group have identified various host cellular proteins that can interact with the Newcastle disease virus V protein (Chu et al., 2018a), but their function in NDV replication has not been fully determined. The present study reports that heterogenous nuclear ribonucleoprotein H1 (hnRNP H1) can interact with NDV V protein in yeast. The immunofluorescence results showed that hnRNP H1 and V protein could colocalize in the cytoplasm of a chicken embryo fibroblast cell line (DF-1 cells). Co-immunoprecipitation assays further verified the interaction of these two proteins. The effects of overexpression and knockdown of hnRNP H1 on NDV replication were evaluated in DF-1 cells through real time quantitative PCR (RT-qPCR) and plaque assays. The regulation of V protein on hnRNP H1 expression was also examined. The results indicated that overexpression of hnRNP H1 facilitated NDV replication, while knockdown of hnRNP H1 decreased NDV replication. It was also shown that V protein could regulate hnRNP H1 expression at the protein level instead of the transcription level. The effect of V protein and hnRNP H1 on the DF-1 cell cycle was also tested and the results revealed that V protein may regulate cell proliferation by controlling the expression of hnRNP H1. Taken together, these results suggest that NDV V protein could promote viral replication by interacting with hnRNP H1.



中文翻译:

新城疫病毒V蛋白与hnRNP H1相互作用促进病毒复制

宿主细胞蛋白和病毒蛋白之间的相互作用对于病毒的成功感染很重要。我们小组之前的研究已经确定了可以与新城疫病毒 V 蛋白相互作用的各种宿主细胞蛋白(Chu 等,2018a),但它们在 NDV 复制中的功能尚未完全确定。本研究报告说,异质核核糖核蛋白 H1 (hnRNP H1) 可以与酵母中的 NDV V 蛋白相互作用。免疫荧光结果显示hnRNP H1和V蛋白可以共定位于鸡胚胎成纤维细胞系(DF-1细胞)的细胞质中。共免疫沉淀分析进一步验证了这两种蛋白质的相互作用。通过实时定量 PCR (RT-qPCR) 和噬斑分析,在 DF-1 细胞中评估了 hnRNP H1 过表达和敲低对 NDV 复制的影响。还检查了 V 蛋白对 hnRNP H1 表达的调节。结果表明,hnRNP H1 的过表达促进了 NDV 复制,而 hnRNP H1 的敲低则减少了 NDV 复制。还表明V蛋白可以在蛋白质水平而不是转录水平上调节hnRNP H1的表达。还测试了V蛋白和hnRNP H1对DF-1细胞周期的影响,结果表明V蛋白可能通过控制hnRNP H1的表达来调节细胞增殖。总之,这些结果表明 NDV V 蛋白可以通过与 hnRNP H1 相互作用来促进病毒复制。还检查了 V 蛋白对 hnRNP H1 表达的调节。结果表明,hnRNP H1 的过表达促进了 NDV 复制,而 hnRNP H1 的敲低则减少了 NDV 复制。还表明V蛋白可以在蛋白质水平而不是转录水平上调节hnRNP H1的表达。还测试了V蛋白和hnRNP H1对DF-1细胞周期的影响,结果表明V蛋白可能通过控制hnRNP H1的表达来调节细胞增殖。总之,这些结果表明 NDV V 蛋白可以通过与 hnRNP H1 相互作用来促进病毒复制。还检查了 V 蛋白对 hnRNP H1 表达的调节。结果表明,hnRNP H1 的过表达促进了 NDV 复制,而 hnRNP H1 的敲低则减少了 NDV 复制。还表明V蛋白可以在蛋白质水平而不是转录水平上调节hnRNP H1的表达。还测试了V蛋白和hnRNP H1对DF-1细胞周期的影响,结果表明V蛋白可能通过控制hnRNP H1的表达来调节细胞增殖。总之,这些结果表明 NDV V 蛋白可以通过与 hnRNP H1 相互作用来促进病毒复制。还表明V蛋白可以在蛋白质水平而不是转录水平上调节hnRNP H1的表达。还测试了V蛋白和hnRNP H1对DF-1细胞周期的影响,结果表明V蛋白可能通过控制hnRNP H1的表达来调节细胞增殖。总之,这些结果表明 NDV V 蛋白可以通过与 hnRNP H1 相互作用来促进病毒复制。还表明V蛋白可以在蛋白质水平而不是转录水平上调节hnRNP H1的表达。还测试了V蛋白和hnRNP H1对DF-1细胞周期的影响,结果表明V蛋白可能通过控制hnRNP H1的表达来调节细胞增殖。总之,这些结果表明 NDV V 蛋白可以通过与 hnRNP H1 相互作用促进病毒复制。

更新日期:2021-07-13
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