Microalgae, a popular source of food and bioactive compounds, accumulate antioxidants in response to culture condition stresses. Using a factorial design (3 × 3), the effect of light, temperature, and nitrogen level on chlorophyll and carotenoids, total protein, total phenolic, ascorbate and glutathione content, and enzyme (catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD)) activities in Dunaliella tertiolecta was studied. Data were analysed using Design of Experiments (DoE), and recommendations are made for optimum cultivation conditions to achieve the highest antioxidant content (phenolics, ascorbate and glutathione) or enzyme (CAT, SOD, and POD) activities. This is the first study to apply three levels of three factors during cultivation to tune Dunaliella tertiolecta for optimal antioxidant production.

微藻是一种流行的食物和生物活性化合物来源，可积累抗氧化剂以应对培养条件压力。使用析因设计 (3 × 3)，光、温度和氮水平对叶绿素和类胡萝卜素、总蛋白、总酚、抗坏血酸和谷胱甘肽含量以及酶（过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、和过氧化物酶 (POD)) 在杜氏盐藻中的活性进行了研究。使用实验设计 (DoE) 分析数据，并针对最佳培养条件提出建议，以实现最高的抗氧化剂含量（酚类、抗坏血酸和谷胱甘肽）或酶（CAT、SOD 和 POD）活性。这是第一项在培养过程中应用三个水平的三个因素来调整杜氏盐藻的研究 以获得最佳的抗氧化剂生产。