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Androgenic Sensitivities and Ovarian Gene Expression Profiles Prior to Treatment in Japanese Eel (Anguilla japonica)
Marine Biotechnology ( IF 3 ) Pub Date : 2021-06-30 , DOI: 10.1007/s10126-021-10035-6
Yung-Sen Huang , Wen-Chih Cheng , Chung-Yen Lin

Androgens stimulate ovarian development in eels. Our previous report indicated a correlation between the initial (debut) ovarian status (determined by kernel density estimation (KDE), presented as a probability density of oocyte size) and the consequence of 17MT treatment (change in ovary). The initial ovarian status appeared to be an important factor influencing ovarian androgenic sensitivity. We postulated that the sensitivities of initial ovaries are correlated with their gene expression profiles. Japanese eels underwent operation to sample the initial ovarian tissues, and the samples were stored in liquid nitrogen. Using high-throughput next-generation sequencing (NGS) technology, ovarian transcriptomic data were mined and analyzed based on functional gene classification with cutoff-based differentially expressed genes (DEGs); the ovarian status was transformed into gene expression profiles globally or was represented by a set of gene list. Our results also implied that the initial ovary might be an important factor influencing the outcomes of 17MT treatments, and the genes related with neuronal activities or neurogenesis seemed to play an essential role in the positive effect.



中文翻译:

日本鳗(Anguilla japonica)治疗前的雄激素敏感性和卵巢基因表达谱

雄激素刺激鳗鱼的卵巢发育。我们之前的报告表明,初始(首次)卵巢状态(由核密度估计 (KDE) 确定,以卵母细胞大小的概率密度表示)与 17MT 治疗的结果(卵巢变化)之间存在相关性。初始卵巢状态似乎是影响卵巢雄激素敏感性的重要因素。我们假设初始卵巢的敏感性与其基因表达谱相关。日本鳗鱼通过手术对初始卵巢组织进行取样,并将样品储存在液氮中。利用高通量二代测序(NGS)技术,基于功能基因分类和基于截止的差异表达基因(DEGs),挖掘和分析卵巢转录组数据;卵巢状态被转化为全局基因表达谱或由一组基因列表表示。我们的结果还表明,初始卵巢可能是影响 17MT 治疗结果的重要因素,与神经元活动或神经发生相关的基因似乎在积极作用中发挥着重要作用。

更新日期:2021-06-30
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