Protein Expression and Purification ( IF 1.6 ) Pub Date : 2021-06-29 , DOI: 10.1016/j.pep.2021.105931 Hee-Jin Jeong 1 , Eun-Jung Kim 2 , Joo-Kyung Kim 1 , Yun-Gon Kim 3 , Chang-Soo Lee 4 , Byoung Joon Ko 5 , Byung-Gee Kim 6
We have successfully produced a recombinant human matrix metalloproteinase 9 (hMMP9) antigen with high yield and purity and used it to generate a hybridoma cell-culture-based monoclonal anti-hMMP9 antibody. We selected the most effective antibody for binding antigens and successfully identified its nucleotide sequence. The entire antigen and antibody developmental procedures described herein can be a practical approach for producing large amounts of monoclonal antibodies against hMMP9 and other antigens of interest. Additionally, the nucleotide sequence information of the anti-hMMP9 monoclonal antibody revealed herein will be useful for the generation of recombinant antibodies or antibody fragments against hMMP9.
中文翻译:
可溶性重组人基质金属蛋白酶9的表达及其单克隆抗体的产生
我们已成功生产出高产率和纯度的重组人基质金属蛋白酶 9 (hMMP9) 抗原,并用它来产生基于杂交瘤细胞培养的单克隆抗 hMMP9 抗体。我们选择了最有效的结合抗原的抗体,并成功鉴定了其核苷酸序列。本文所述的整个抗原和抗体开发程序可以是用于生产大量针对 hMMP9 和其他感兴趣抗原的单克隆抗体的实用方法。此外,本文揭示的抗hMMP9单克隆抗体的核苷酸序列信息将可用于产生针对hMMP9的重组抗体或抗体片段。