Rationale

Despite a long history of use in synaptic physiology, the lobster has been a neglected model for behavioral pharmacology. A restaurateur proposed that exposing lobster to cannabis smoke reduces anxiety and pain during the cooking process. It is unknown if lobster gill respiration in air would result in significant Δ⁹-tetrahydrocannabinol (THC) uptake and whether this would have any detectable behavioral effects.

Objective

The primary goal was to determine tissue THC levels in the lobster after exposure to THC vapor. Secondary goals were to determine if THC vapor altered locomotor behavior or nociception.

Methods

Tissue samples were collected (including muscle, brain and hemolymph) from Homarus americanus (N = 3 per group) following 30 or 60 min of exposure to vapor generated by an e-cigarette device using THC (100 mg/mL in a propylene glycol vehicle). Separate experiments assessed locomotor behavior and hot water nociceptive responses following THC vapor exposure.

Results

THC vapor produced duration-related THC levels in all tissues examined. Locomotor activity was decreased (distance, speed, time-mobile) by 30 min inhalation of THC. Lobsters exhibit a temperature-dependent withdrawal response to immersion of tail, antennae or claws in warm water; this is novel evidence of thermal nociception for this species. THC exposure for 60 min had only marginal effect on nociception under the conditions assessed.

Conclusions

Vapor exposure of lobsters, using an e-cigarette based model, produces dose-dependent THC levels in all tissues and reduces locomotor activity. Hot water nociception was temperature dependent, but only minimal anti-nociceptive effect of THC exposure was confirmed.

中文翻译：

---

蒸气暴露于 Δ9-四氢大麻酚 (THC) 会减慢缅因州龙虾 (Homarus americanus) 的运动

基本原理

尽管在突触生理学中的使用历史悠久，但龙虾一直是行为药理学被忽视的模型。一位餐馆老板建议将龙虾暴露在大麻烟中可以减少烹饪过程中的焦虑和疼痛。目前尚不清楚龙虾鳃在空气中的呼吸是否会导致显着的 Δ ⁹ -四氢大麻酚 (THC) 摄取，以及这是否会产生任何可检测的行为影响。

客观的

主要目标是确定暴露于 THC 蒸气后龙虾中的组织 THC 水平。次要目标是确定 THC 蒸气是否改变了运动行为或伤害感受。

方法

在暴露于使用 THC 的电子烟装置产生的蒸汽 30 或 60 分钟后，从美洲白鹤（每组N = 3 ）收集组织样本（包括肌肉、大脑和血淋巴） （ 在丙二醇载体中为 100 mg/mL） ）。单独的实验评估了 THC 蒸汽暴露后的运动行为和热水伤害性反应。

结果

THC 蒸气在所有检查的组织中产生与持续时间相关的 THC 水平。吸入四氢大麻酚 30 分钟后，运动活动（距离、速度、时间移动）降低。龙虾在将尾巴、触角或爪子浸入温水中时会表现出与温度有关的退缩反应；这是该物种热伤害感受的新证据。在评估的条件下，THC 暴露 60 分钟对伤害感受只有边际效应。

结论

使用基于电子烟的模型，龙虾的蒸汽暴露会在所有组织中产生剂量依赖性 THC 水平并降低运动活动。热水伤害感受与温度有关，但仅证实了 THC 暴露的最小抗伤害作用。