MicroRNA-155 (miRNA-155) as a characteristic myeloma-associated biomarker exhibits significant potential application in the diagnosis of multiple myeloma (MM). In this paper, a novel type of molecular beacon (MB)-functionalized monolayer MoS₂ nanosheet probe was proposed as fluorescent probe for high-sensitive assays of miRNA-155that uses a duplexspecificnuclease (DSN) enzyme to amplify the fluorescence signal. The preparation and detection conditions of the fluorescent probes were optimized in some aspects, such as the concentration of MoS₂ (0.80 μM) and DSN (0.2 U), and the incubation time of DSN (30 min). The probesexhibited a sensitive fluorescence response to miRNA-155 and the fluorescence signal of the assay was significantly amplified by the cleavage of DSN. The relationship between F/F₀ and logC miRNA follows a linear calibration curve, and the limit of detection (LOD) of miRNA-155 in 10% human serum is calculated to be 10.96 fM based on this relationship. The good performance and fluorescence amplification effect of the fluorescent probe were confirmed by studying the recovery of miRNA-155 in 10% human serum, which was ranged from 98.32% to 106.3% with a relative standard deviation of less than 4.14%. Besides, the high expression of miRNA-155 in clinic blood of MM patients was sensitively distinguished from healthy peoples by using the proposed probes. The proposed novel fluorescent probe based on the DSN can be used to detect miRNA-155 in human serum and provide a potential, convenient and reliable tool for diagnosis of MM.

中文翻译：

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使用分子信标功能化单层二硫化钼纳米片探针和双链特异性核酸酶介导的信号放大，高灵敏度检测 miRNA-155。

MicroRNA-155 (miRNA-155) 作为一种特征性的骨髓瘤相关生物标志物，在多发性骨髓瘤 (MM) 的诊断中具有重要的潜在应用价值。在本文中，提出了一种新型分子信标 (MB) 功能化单层二硫化钼纳米片探针作为 miRNA-155 高灵敏度测定的荧光探针，该探针使用双链特异性核酸酶 (DSN) 酶来放大荧光信号。荧光探针的制备和检测条件在MoS2 (0.80 μ M)和DSN (0.2 U)的浓度、DSN的孵育时间(30 min)等方面进行了优化。探针对 miRNA-155 表现出敏感的荧光反应，并且 DSN 的裂解显着放大了检测的荧光信号。F/F之间的关系₀和 logC miRNA 遵循线性校准曲线，基于这种关系，10% 人血清中 miRNA-155 的检测限 (LOD) 计算为 10.96 fM。通过研究10%人血清中miRNA-155的回收率，证实了该荧光探针的良好性能和荧光放大效果，回收率范围为98.32%~106.3%，相对标准偏差小于4.14%。此外，通过使用所提出的探针，可以敏感地区分 MM 患者临床血液中 miRNA-155 的高表达与健康人群。所提出的基于 DSN 的新型荧光探针可用于检测人血清中的 miRNA-155，为 MM 的诊断提供一种潜在、方便和可靠的工具。