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Nanotopography-Modulated Epithelial Cell Collective Migration.
Journal of Biomedical Nanotechnology ( IF 2.9 ) Pub Date : 2021-6-26 , DOI: 10.1166/jbn.2021.3086
Zaozao Chen 1 , Qiwei Li 1 , Shihui Xu 1 , Jun Ouyang 2 , Hongmei Wei 1
Affiliation  

Matrix nanotopography plays an essential role in regulating cell behaviors including cell proliferation, differentiation, and migration. While studies on isolated single cell migration along the nanostructural orientation have been reported for various cell types, there remains a lack of understanding of how nanotopography regulates the behavior of collectively migrating cells during processes such as epithelial wound healing. We demonstrated that collective migration of epithelial cells was promoted on nanogratings perpendicular to, but not on those parallel to, the wound-healing axis. We further discovered that nanograting-modulated epithelial migration was dominated by the adhesion turnover process, which was Rho-associated protein kinase activity-dependent, and the lamellipodia protrusion at the cell leading edge, which was Rac1-GTPase activity-dependent. This work provides explanations to the distinct migration behavior of epithelial cells on nanogratings, and indicates that the effect of nanotopographic modulations on cell migration is cell-type dependent and involves complex mechanisms.

中文翻译:

Nanotopography-Modulated 上皮细胞集体迁移。

基质纳米拓扑结构在调节细胞行为(包括细胞增殖、分化和迁移)中起着至关重要的作用。虽然已经报道了对各种细胞类型沿纳米结构方向的孤立单细胞迁移的研究,但仍然缺乏对纳米拓扑如何在上皮伤口愈合等过程中调节集体迁移细胞行为的了解。我们证明上皮细胞的集体迁移在与伤口愈合轴垂直但平行于伤口愈合轴的纳米格栅上得到促进。我们进一步发现,纳米光栅调节的上皮迁移主要由黏附周转过程主导,这是 Rho 相关蛋白激酶活性依赖的,以及细胞前沿的片状伪足突起,这是 Rac1-GTPase 活性依赖性的。这项工作解释了上皮细胞在纳米光栅上的不同迁移行为,并表明纳米拓扑调制对细胞迁移的影响取决于细胞类型并涉及复杂的机制。
更新日期:2021-06-30
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