Oral squamous cell carcinoma (OSCC) is one of the most common tumors worldwide and has one of the highest mortalities. The progression of OSCC is accompanied by changes in the levels of many genes. Iroquois homeobox 5 (IRX5), a novel protein involved in several embryonic developmental processes, has been found in recent years to play a significant role in regulating the growth of malignant tumors. However, its role and mechanism in OSCC are still unclear. In this study, we used nano-PCR to examine the levels of IRX5 in OSCC tissues. Through overexpression and knockdown experiments, we researched the role of IRX5 in regulating OSCC cell multiplication, metastasis, and epithelial-mesenchymal transition (EMT). The results demonstrated that IRX5 expression is higher in OSCC tissues in contrast to adjacent tissues. Overexpression of IRX5 promotes the multiplication, metastasis, invasion, and EMT of OSCC cells. Additional bioinformatics analysis showed that miRNA-147 can target the 3'UTR end of IRX5 and negatively regulate its expression, and overexpression of miRNA-147 can weaken the cancer-promoting effect of IRX5. In conclusion, this study found that IRX5 plays a role in promoting cancer in OSCC, and IRX5 is also negatively regulated by miRNA-147.

中文翻译：

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由 miRNA-147 负调控的 Iroquois Homeobox 5 促进口腔鳞状细胞癌的增殖、转移和侵袭。

口腔鳞状细胞癌 (OSCC) 是全球最常见的肿瘤之一，也是死亡率最高的肿瘤之一。OSCC的进展伴随着许多基因水平的变化。易洛魁同源框 5 (IRX5) 是一种参与多种胚胎发育过程的新型蛋白质，近年来被发现在调节恶性肿瘤的生长中发挥重要作用。然而，其在 OSCC 中的作用和机制仍不清楚。在这项研究中，我们使用纳米 PCR 来检查 OSCC 组织中 IRX5 的水平。通过过表达和敲低实验，我们研究了 IRX5 在调节 OSCC 细胞增殖、转移和上皮间质转化 (EMT) 中的作用。结果表明，与邻近组织相比，IRX5 在 OSCC 组织中的表达更高。IRX5的过表达促进OSCC细胞的增殖、转移、侵袭和EMT。额外的生物信息学分析表明，miRNA-147可以靶向IRX5的3'UTR末端并负调控其表达，而miRNA-147的过表达会削弱IRX5的促癌作用。综上所述，本研究发现IRX5在OSCC中具有促癌作用，并且IRX5也受miRNA-147的负调控。