Two novel metal–organic coordination complexes based on L [L = 3,6-bis(imidazol-1-yl)pyridazine], which could also be called as [Ni(L)(1,3,5-H₂BTC)₂H₂O]_n 1 (1,3,5-H₃BTC = 1,3,5-benzenetricarboxylic acid) and {[Ni₂(L)(2,6-PYDC)₂(H₂O)₂]·5H₂O}_n 2 (2,6-H₂PYDC = 2,6-pyridine dicarboxylic acid) have been formed using solvothermal method by reaction of Ni(II) salts with the L ligand in the presence of different carboxylate ligands (1,3,5-H₃BTC for 1 and 2,6-H₂PYDC for 2). The treatment activity of compounds 1 and 2 on the neonatal hypoxic-ischemic encephalopathy was estimated, then the relevant mechanism was discussed. Firstly, the quantity of the tumor necrosis factor-α (TNF-α) and neuron-specific enolase (NSE) in the cerebrospinal fluid was measured by enzyme linked immunosorbent assay (ELISA) detection kit. In addition to this, the expression levels of the glutamate receptor on the brain nerve cells were estimated by real time reverse transcription-polymerase chain reaction (RT-PCR).

两种基于 L [L = 3,6-双(咪唑-1-基)哒嗪]的新型金属-有机配位配合物，也可称为 [Ni(L)(1,3,5-H ₂ BTC) ₂ H ₂ O] _n 1 (1,3,5-H ₃ BTC = 1,3,5-苯三甲酸) 和 {[Ni ₂ (L)(2,6-PYDC) ₂ (H ₂ O) ₂ ] ·5H ₂ O} _n 2 (2,6-H ₂ PYDC = 2,6-吡啶二羧酸) 使用溶剂热法通过 Ni(II) 盐与 L 配体在不同羧酸盐配体的存在下反应形成（ 1,3,5-H ₃ BTC 用于1和 2,6-H₂ PYDC 为2 )。评估了化合物1和2对新生儿缺氧缺血性脑病的治疗活性，并讨论了相关机制。首先，采用酶联免疫吸附试验（ELISA）检测试剂盒测定脑脊液中肿瘤坏死因子-α（TNF-α）和神经元特异性烯醇化酶（NSE）的含量。除此之外，脑神经细胞上谷氨酸受体的表达水平通过实时逆转录聚合酶链反应 (RT-PCR) 进行评估。