Chlorpyrifos is a broad-spectrum organophosphate pesticide and used frequently on different cropping systems to control phytopathogens. Being a hazardous substance, chlorpyrifos may affect human health as some unutilized pesticide percolates through soil and contaminates ground water. Present study was planned to establish an effective remediation method of chlorpyrifos using two fungal isolates (C1 and C3), recovered from the waste (soil) of a refrigeration industry. C1 and C3, identified as Byssochlamys spectabilis and Aspergillus fumigates respectively were able to tolerate 600 mg L^− 1 of chlorpyrifos. “The Half-life of chlorpyrifos (control) was 231 days which could be reduced to 13.6 days” under in vitro condition in the presence of mixture of two fungi in Czapek dox medium (CDM). Consortium of the fungi showed 98.4 % degradation of chlorpyrifos within 30 days in CDM. Degradation of the pesticide was related to specific laccase activity of the fungi and the level of enzyme activity was better in consortium 9.09 and 10.29 U mL^− 1 after 6 and 10 days respectively. Immobilized fungal enzymes also showed appreciable biodegradation of the pesticide by providing extra matrix for enzyme substrate reaction. Residual analysis of chlorpyrifos in the soil of onion (Allium cepa) in a phytoremediation experiment revealed a significant decrease in t_1/2 value in the presence of fungi. Hence, it is concluded that chlorpyrifos biodegradation ability of fungal consortium reveals the potential of these isolates in biodegradation of toxic compounds from contaminated water and soil. However, further studies are needed to access the biodegradation of the pesticide in different soil types, crops and under varied environmental conditions at molecular level.

毒死蜱是一种广谱有机磷农药，经常用于不同的种植系统以控制植物病原体。作为一种有害物质，毒死蜱可能会影响人类健康，因为一些未使用的农药会渗入土壤并污染地下水。本研究计划使用从制冷行业的废物（土壤）中回收的两种真菌分离物（C1 和 C3）建立一种有效的毒死蜱修复方法。C1 和 C3，分别被鉴定为Byssochlamys spectabilis和Aspergillus fumigates，能够耐受 600 mg L ^{- 1}的毒死蜱。“毒死蜱（对照）的半衰期为 231 天，可缩短至 13.6 天”在体外在 Czapek dox 培养基 (CDM) 中存在两种真菌混合物的条件。在 CDM 中，真菌聚生体在 30 天内降解了 98.4% 的毒死蜱。杀虫剂的降解与真菌的特定漆酶活性有关，并且酶活性水平在 9.09 和 10.29 U mL ^{- 1 的}菌群中分别在 6 天和 10 天后更好。通过为酶底物反应提供额外的基质，固定的真菌酶也显示出可观的农药生物降解。在植物修复实验中对洋葱 ( Allium cepa )土壤中毒死蜱的残留分析表明 t _1/2显着降低。存在真菌时的价值。因此，可以得出结论，真菌聚生体的毒死蜱生物降解能力揭示了这些分离物在生物降解受污染水和土壤中有毒化合物方面的潜力。然而，需要进一步研究以在分子水平上了解农药在不同土壤类型、作物和不同环境条件下的生物降解情况。