The development of mycological gerontology requires effective methods for assessing the biological age of fungal cells. This assessment is based on the analysis of a complex of aging and oxidative stress markers. One of the most powerful such markers is the protein carbonylation. In this study, the already known method of dry immune dot blotting is adapted for mycological studies of the content of protein carbonyl groups. After testing the method on a number of filamentous fungi species, some features of the accumulation of carbonylated proteins in mycelium were established. Among these features: (i) a weak effect of exogenous oxidative stress on the accumulation of carbonyls in a number of fungi, (ii) reversibility of the carbonyl accumulation, (iii) possibility of arbitrary regulation of carbonyl content by fungus itself and (iv) the influence of hormesis. In addition, two polar strategies for the accumulation of carbonyl modification were revealed, named Id-strategy (Indifferent) and Cn-strategy (Concern). Thus, even the analysis of one marker allows making some preliminary general assumptions and conclusions. For example, the idea that fungi can freely regulate their biological age is confirmed. This feature makes fungi very flexible in terms of responding to environmental influences and promising objects for gerontology.

真菌老年学的发展需要有效的方法来评估真菌细胞的生物学年龄。该评估基于对衰老和氧化应激标志物复合物的分析。最强大的此类标记之一是蛋白质羰基化。在这项研究中，已知的干免疫斑点印迹方法适用于蛋白质羰基含量的真菌学研究。在对许多丝状真菌物种进行测试后，确定了菌丝体中羰基化蛋白质积累的一些特征。在这些特征中：（i）外源性氧化应激对许多真菌中羰基化合物的积累的影响较弱，（ii）羰基积累的可逆性，(iii) 真菌本身任意调节羰基含量的可能性和 (iv) 兴奋作用的影响。此外，揭示了两种用于积累羰基修饰的极性策略，称为 Id 策略（无所谓）和 Cn 策略（关注）。因此，即使是对一个标记的分析也允许做出一些初步的一般假设和结论。例如，真菌可以自由调节其生物学年龄的想法得到证实。这一特征使真菌在应对环境影响和老年学有希望的对象方面非常灵活。甚至对一个标记的分析也允许做出一些初步的一般假设和结论。例如，真菌可以自由调节其生物学年龄的想法得到证实。这一特征使真菌在应对环境影响和老年学有希望的对象方面非常灵活。甚至对一个标记的分析也允许做出一些初步的一般假设和结论。例如，真菌可以自由调节其生物学年龄的想法得到证实。这一特征使真菌在应对环境影响和老年学有希望的对象方面非常灵活。