In this study, the medium requirements to increase the production of xylitol by using Candida tropicalis (CT) have been investigated. The technique of single addition or omission of medium components was applied to determine the nutritional requirements. The addition of amino acids such as Asp, Glu, Gln, Asn, Thr, and Gly had no significant effect on CT growth. However, in the absence of other metal ions, there was a higher concentration of cell growth and xylitol production when only Zn²⁺ was present in the medium. The analysis of various vitamins unveiled that biotin and thiamine were the only vitamins required for the growth of CT. Surprisingly, when only biotin was present in the medium as a vitamin, there was less growth of CT than when the medium was complete, but the amount of xylitol released was significantly higher. Overall, this study will increase the xylitol production using the single omission or addtion technique.

在本研究中，研究了使用热带念珠菌(CT)增加木糖醇产量的培养基需求。采用单次添加或省略培养基成分的技术来确定营养需求。Asp、Glu、Gln、Asn、Thr和Gly等氨基酸的添加对CT生长没有显着影响。然而，在没有其他金属离子的情况下，只有 Zn ^{2+时，细胞生长和木糖醇产生的浓度更高。}存在于介质中。对各种维生素的分析表明，生物素和硫胺素是 CT 生长所需的唯一维生素。令人惊讶的是，当培养基中仅存在生物素作为维生素时，CT 的生长比培养基完全时少，但释放的木糖醇量明显更高。总体而言，这项研究将使用单一省略或添加技术增加木糖醇的产量。